Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans.
Toxicity evaluation of boron nitride nanospheres and water-soluble boron nitride in Caenorhabditis elegans.
复制标题
氮化硼纳米球和水溶性氮化硼对秀丽隐杆线虫的毒性评价
DOI：
10.2147/ijn.s130960
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发表时间：
2017
影响因子：
8
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Wang N;Wang H;Tang C;Lei S;Shen W;Wang C;Wang G;Wang Z;Wang L
氮化硼（BN）纳米材料在生物领域的潜在应用已得到越来越多的探索。然而，人们对它们的毒性仍然知之甚少。利用秀丽隐杆线虫作为全动物模型，对两种具有代表性的氮化硼纳米球（BNNSs）和高水溶性氮化硼纳米材料（BN -800-2）进行毒性分析，发现BNNSs的总体毒性低于形状不规则的可溶性BN-800-2。BNNSs和BN-800-2的浓度阈值分别为100µg·mL - 1和10µg·mL - 1。高于此浓度，生长迟缓，寿命缩短，后代减少，运动行为迟缓，表型相关基因表达不同程度地改变。BNNSs和BN-800-2通过促进活性氧的产生而增加秀丽隐杆线虫的氧化应激水平。我们的研究结果进一步表明，氧化应激反应和MAPK信号相关基因，如GAS1、SOD2、SOD3、MEK1和PMK1，可能是对BNNSs和BN-800-2暴露的活性氧产生和毒性反应的关键因素。综上所述，我们的研究结果表明，当浓度低于10µg·mL−1时，BNNSs比BN-800-2具有更强的生物相容性，是潜在的生物相容性材料。
Boron nitride (BN) nanomaterials have been increasingly explored for potential biological applications. However, their toxicity remains poorly understood. Using Caenorhabditis elegans as a whole-animal model for toxicity analysis of two representative types of BN nanomaterials – BN nanospheres (BNNSs) and highly water-soluble BN nanomaterial (named BN-800-2) – we found that BNNSs overall toxicity was less than soluble BN-800-2 with irregular shapes. The concentration thresholds for BNNSs and BN-800-2 were 100 µg·mL−1 and 10 µg·mL−1, respectively. Above this concentration, both delayed growth, decreased life span, reduced progeny, retarded locomotion behavior, and changed the expression of phenotype-related genes to various extents. BNNSs and BN-800-2 increased oxidative stress levels in C. elegans by promoting reactive oxygen species production. Our results further showed that oxidative stress response and MAPK signaling-related genes, such as GAS1, SOD2, SOD3, MEK1, and PMK1, might be key factors for reactive oxygen species production and toxic responses to BNNSs and BN-800-2 exposure. Together, our results suggest that when concentrations are lower than 10 µg·mL−1, BNNSs are more biocompatible than BN-800-2 and are potentially biocompatible material.