Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase
Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase
复制标题
利用肽嵌入式雷尼拉荧光素酶直接检测活细胞中的 RNA
DOI:
10.1039/c1an15130d
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发表时间:
2011-01-01
期刊:
影响因子:
4.2
通讯作者:
Kobatake, Eiry
中科院分区:
文献类型:
--
作者:
Andou, Takashi;Endoh, Tamaki;Kobatake, Eiry
In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.