Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase

Direct detection of RNAs in living cells using peptide-inserted Renilla luciferase
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利用肽嵌入式雷尼拉荧光素酶直接检测活细胞中的 RNA

DOI:
10.1039/c1an15130d
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发表时间:
2011-01-01
期刊:
影响因子:
4.2
通讯作者:
Kobatake, Eiry
Kobatake, Eiry
中科院分区:
化学2区
文献类型:
--
作者:
Andou, Takashi;Endoh, Tamaki;Kobatake, Eiry

文献摘要

被引文献

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在这项研究中,利用生物发光技术在活细胞中检测到了非工程RNA。使用了两种类型的探针:插入多肽的RLuc(PI-RLuc)探针和裂解RNA探针。PI-RLuc和Split-RNA探针的掺入使直接检测到导入活细胞的RNA成为可能。
In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.