In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis
In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilis
复制标题
原位杂交鉴定淡水海绵 Ephydatia Fluviatilis 中的干细胞
DOI：
10.1007/978-1-0716-2172-1_17
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发表时间：
2022
影响因子：
--
通讯作者：
Funayama Noriko
中科院分区：
文献类型：
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作者：
Kojima Chiaki;Funayama Noriko
海绵（多孔动物）是一个大的门，包括大量的物种。他们被分为四类。在这四个纲中，Demospongiae纲是最大的，包含超过90%的海绵物种。在过去的十年中，海绵生物学中的分子研究和测序资源的方法学已经取得了显着的进步，并且可以根据它们所表达的基因来明确定义特定类型的细胞。本文详细介绍了我们建立的高分辨率原位杂交（whole mount in situ hybridization，WISH）和双色荧光原位杂交（dual color fluorescence detection of situ hybridization，Dual color FISH）方法，用于检测淡水海绵E.河流
Sponges (Porifera) are a large phylum that includes an enormous number of species. They are classified into four classes. Among these four classes, class Demospongiae is the largest and contains more than 90% of sponge species. In the last decade, methodologies for molecular studies and sequencing resources in sponge biology have dramatically advanced and made it possible to clearly define particular types of cells based on the genes they are expressing. Here we describe in detail the method of high-resolution WISH (whole mount in situ hybridization) and dual color fluorescent detection of in situ hybridization (dual color FISH) that we have established to detect particular types of cells, especially their stem cells known as archeocytes, in juveniles of freshwater demosponge, E. fluviatilis.