Biaxial strength of multilaminated extracellular matrix scaffolds
Biaxial strength of multilaminated extracellular matrix scaffolds
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DOI：
10.1016/j.biomaterials.2003.09.015
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发表时间：
2004-05-01
期刊：
影响因子：
14
通讯作者：
Rundell, AE
中科院分区：
文献类型：
--
作者：
Freytes, DO;Badylak, SF;Rundell, AE
异种细胞外基质（ECM）可以被收获并配置为用作组织和器官重建的生物支架。ECM的机械性质根据其所收获的组织而变化。同样，将ECM开发成医用级器械所需的制造步骤将影响生物支架的表面形态和机械性能;这是建设性组织重塑的重要性能。本研究比较了五种不同ECM支架在用过氧乙酸（PAA）处理之前和之后的球爆裂强度：猪小肠粘膜下层（SIS）、猪膀胱粘膜下层（UBS）、猪膀胱基质（UBM）、UBS + UBM的复合物和犬胃粘膜下层（SS）。本研究还比较了2层和4层ECM支架的机械性能。结果表明，2层SS器械在所有2层ECM器械中具有最高的钢球顶破值。此外，除了4层UBM器械最弱外，所有4层ECM器械均具有相似的球顶强度。PAA处理降低了SS的球顶强度，并增加了UBS 2层器件的球顶强度。该研究表明，通过改变层数和修改用于制造的消毒/灭菌处理，ECM支架的材料特性可以被工程化以模拟天然软组织（即血管、肌肉肌腱等）的材料特性。(C)2003 Elsevier Ltd.保留所有权利。
Xenogeneic extracellular matrix (ECM) can be harvested and configured to function as a bioscaffold for tissue and organ reconstruction. The mechanical properties of the ECM vary depending upon the tissue from which it is harvested. Likewise, the manufacturing steps required to develop ECMs into medical grade devices will affect the surface morphology and the mechanical properties of the bioscaffold; important properties for constructive tissue remodeling. The present study compared the ball-burst strength of five different ECM scaffolds before and after treatment with peracetic acid (PAA): porcine small intestinal submucosa (SIS), porcine urinary bladder submucosa (UBS), porcine urinary bladder matrix (UBM), a composite of UBS + UBM, and canine stomach submucosa (SS). This study also compared the mechanical properties of 2- and 4-layer ECM scaffolds. Results showed 2-layer SS devices had the highest ball-burst value of all 2-layer ECM devices. Moreover, all 4-layer ECM devices had similar ball-burst strength except for 4-layer UBM devices which was the weakest. PAA-treatment decreased the ball-burst strength of SS and increased the ball-burst strength of UBS 2-layer devices. This study showed the material properties of the ECM scaffolds could be engineered to mimic those of native soft tissues (i.e. vascular, musculotendinous, etc) by varying the number of layers and modifying the disinfection/sterilization treatments used for manufacturing. (C) 2003 Elsevier Ltd. All rights reserved.