Hydraulic fracturing and active coarsening position the lumen of the mouse blastocyst
Hydraulic fracturing and active coarsening position the lumen of the mouse blastocyst
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DOI：
10.1126/science.aaw7709
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发表时间：
2019-08-02
期刊：
影响因子：
56.9
通讯作者：
Maitre, Jean-Leon
中科院分区：
文献类型：
--
作者：
Dumortier, Julien G.;Le Verge-Serandour, Mathieu;Maitre, Jean-Leon
在小鼠着床前发育过程中，囊胚腔（充满液体的腔）的形成打破了囊胚的径向对称性。控制该基底外侧腔的形成和定位的因素仍然不清楚。我们发现，加压流体的积累使细胞-细胞接触破裂成数百微米大小的管腔。这些微腔最终将它们的体积排放到一个单一的主导腔中，我们将其建模为类似于奥斯特瓦尔德成熟的过程，这是泡沫粗化的基础。使用嵌合突变胚胎，我们调整了细胞-细胞接触的水力压裂，并控制了微腔的粗化，使我们能够成功地操纵管腔的最终位置。我们的结论是，水力压裂的细胞-细胞接触，然后由收缩导向粗化的微腔设置的第一个对称轴的小鼠胚胎。
During mouse pre-implantation development, the formation of the blastocoel, a fluid-filled lumen, breaks the radial symmetry of the blastocyst. The factors that control the formation and positioning of this basolateral lumen remain obscure. We found that accumulation of pressurized fluid fractures cell-cell contacts into hundreds of micrometer-size lumens. These microlumens eventually discharge their volumes into a single dominant lumen, which we model as a process akin to Ostwald ripening, underlying the coarsening of foams. Using chimeric mutant embryos, we tuned the hydraulic fracturing of cell-cell contacts and steered the coarsening of microlumens, allowing us to successfully manipulate the final position of the lumen. We conclude that hydraulic fracturing of cell-cell contacts followed by contractility-directed coarsening of microlumens sets the first axis of symmetry of the mouse embryo.