Deficient in DNA methylation 1 (DDM1) defines a novel family of chromatin-remodeling factors
Deficient in DNA methylation 1 (DDM1) defines a novel family of chromatin-remodeling factors
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DOI：
10.1074/jbc.m209260200
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发表时间：
2003-01-10
影响因子：
4.8
通讯作者：
Jerzmanowski, A
中科院分区：
文献类型：
--
作者：
Brzeski, J;Jerzmanowski, A
DNA甲基化1（DDM 1）蛋白的缺失是维持拟南芥DNA甲基化状态所必需的。DNF是广泛的SW 12/SNF 2蛋白家族的成员。由于其系统发育的位置，DALPHINE已被推测作为一个染色质重塑因子。在这里，我们使用一个纯化的重组DDM 1蛋白，以调查是否可以在体外重塑染色质。我们表明，DAPs是一个ATP酶刺激的裸和核小体DNA。DDM 1与核小体结合并以ATP依赖性方式促进染色质重塑。具体而言，它诱导核小体在短DNA片段上重新定位。DDM 1的酶活性不受DNA甲基化的影响。这些研究结果的相关性DDM 1在体内的作用进行了讨论。
Deficient in DNA Methylation 1 (DDM1) protein is required to maintain the DNA methylation status of Arabidopsis thaliana. DDMI is a member of the broad SW12/SNF2 protein family. Because of its phylogenetic position, DDMI has been speculated to act as a chromatin-remodeling factor. Here we used a purified recombinant DDM1 protein to investigate whether it can remodel chromatin in vitro. We show that DDMI is an ATPase stimulated by both naked and nucleosomal DNA. DDM1 binds to the nucleosome and promotes chromatin remodeling in an ATP-dependent manner. Specifically, it induces nucleosome repositioning on a short DNA fragment. The enzymatic activity of DDM1 is not affected by DNA methylation. The relevance of these findings to the in vivo role of DDM1 is discussed.