Analysis of Drug-Resistant Strains of Mycobacterium leprae in an Endemic Area of Vietnam
Analysis of Drug-Resistant Strains of Mycobacterium leprae in an Endemic Area of Vietnam
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DOI：
10.1093/cid/ciq217
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发表时间：
2011-03-01
影响因子：
11.8
通讯作者：
Thanh Tan Nguyen
中科院分区：
文献类型：
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作者：
Kai, Masanori;Nhu Ha Nguyen Phuc;Thanh Tan Nguyen
背景。多种药物治疗有效地减少了世界上麻风病病例的数量。然而，近年来发病率下降的速度有所放缓，这使得麻风分枝杆菌的早期检测和复发的流行病学研究在试图消除麻风分枝杆菌方面具有新的意义。对越南中部和高原地区耐药麻风分枝杆菌进行了分子流行病学调查。通过对麻风分枝杆菌对氨苯砜、利福平和氧氟沙星耐药基因folP1、rpoB和gyrA的耐药决定区进行测序，对83例新发病例、321例正在接受治疗的患者和19例复发患者共423份样本进行耐药突变检测。在folP1基因样本中发现了19个突变，在rpoB和gyrA基因中均未发现与耐药相关的突变。复发患者的样本显示folP1突变率高达57%，新病例和近期病例样本的突变率为
Background. Multidrug therapy has effectively reduced the number of leprosy cases in the world. However, the rate of reduction has decelerated over the years, giving early detection of Mycobacterium leprae and epidemiological study of relapse renewed relevance in attempts to eliminate the disease.Methods. A molecular epidemiological survey for drug-resistant M. leprae was conducted in the central and highland regions of Vietnam. A total of 423 samples taken from patients, including 83 patients with new cases, 321 patients receiving treatment, and 19 patients with relapse, were studied for detection of M. leprae with mutations relating to drug resistance by sequencing the drug resistance determining region of the folP1, rpoB, and gyrA genes, which are responsible for dapsone, rifampicin, and ofloxacin resistance, respectively.Results. Nineteen mutations were found in the folP1 gene samples, and no mutations relating to drug resistance were found in either the rpoB or gyrA genes. Samples from patients with relapse showed folP1 mutation rates as high as 57%, and the mutation rates in samples from new and recent cases were