Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity.
Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity.
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Ly-6 在淋巴细胞激活中的作用。
DOI：
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发表时间：
1986
影响因子：
4.4
通讯作者：
Thomas R. Malek
中科院分区：
文献类型：
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作者：
G. Ortega;P. Korty;Ethan M. Shevach;Thomas R. Malek
在使用同种抗血清和单克隆抗体（mAb）的研究中，已经确定了许多抗原决定簇，它们是鼠2号染色体上Ly-6位点的产物，主要在B和T淋巴细胞上表达。这些抗原决定簇是由单基因还是多基因复合体编码仍有争议。我们的特点是一种新的大鼠单克隆抗体，D 7，它识别的细胞表面抗原，其表达的非活化外周淋巴细胞各不相同的应变。染色谱的表型，即，通过菌株分布研究、RI系和Ly-6同源菌株测定，定位于Ly-6基因座的D 7阳性细胞百分比较高或较低。针对Ly-6E.1特异性的mAb可抑制D 7与Ly-6.1阳性菌株的结合，而D 7可抑制针对Ly-6A.2特异性的mAb与来自Ly-6.2阳性菌株的细胞的结合。共沉淀研究，然后进行蛋白质印迹分析证实，D 7与Ly-6E.1-和Ly-6A.2-轴承分子反应。这些发现最可能的解释是Ly-6A.2和Ly-6E.1代表等位基因特异性。进一步解剖的Ly-6抗原系统的复杂性，并确定其可能的功能在淋巴细胞活化的重要性，应大大促进了异种单克隆抗体，识别多个Ly-6产品的框架决定簇的可用性。
In studies with alloantisera and monoclonal antibodies (mAb) a number of antigenic determinants have been defined that are the products of the Ly-6 locus on murine chromosome 2 and that are expressed primarily on B and T lymphoid cells. It remains controversial whether these antigenic determinants are encoded by a single gene or a multigene complex. We have characterized a new rat mAb, D7, which recognizes a cell surface antigen whose expression on nonactivated peripheral lymphocytes varies from strain to strain. The phenotype of the staining profile, i.e., high or low percentage of D7-positive cells, mapped to the Ly-6 locus as assayed by strain distribution studies, RI lines, and Ly-6 congenic strains. The binding of D7 to Ly-6.1-positive strains could be inhibited by mAb directed to the Ly-6E.1 specificity, whereas D7 could inhibit the binding of mAb specific for Ly-6A.2 to cells from Ly-6.2-positive strains. Coprecipitation studies followed by Western blot analysis confirmed that D7 reacts with both Ly-6E.1- and Ly-6A.2-bearing molecules. The most likely explanation for these findings is that Ly-6A.2 and Ly-6E.1 represent allelic specificities. Further dissection of the complexity of the Ly-6 antigen system and determination of its possible functional importance in lymphocyte activation should be greatly facilitated by the availability of xenogeneic mAb that recognize framework determinants on multiple Ly-6 products.