Protective Effects of 3,4-Oxo-isopropylidene-Shikimic Acid on Experimental Colitis Induced by Trinitrobenzenesulfonic Acid in Rats
Protective Effects of 3,4-Oxo-isopropylidene-Shikimic Acid on Experimental Colitis Induced by Trinitrobenzenesulfonic Acid in Rats
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3,4-氧代异丙叉莽草酸对三硝基苯磺酸所致大鼠实验性结肠炎的保护作用
DOI：
10.1007/s10620-012-2155-y
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发表时间：
2012-08-01
影响因子：
3.1
通讯作者：
Dong, Ya-Lin
中科院分区：
文献类型：
--
作者：
Xing, Jian-Feng;Sun, Jian-Ning;Dong, Ya-Lin
背景：3，4-氧代异亚丙基莽草酸（伊萨）是莽草酸（SA）的衍生物。SA是从八角茴香中提取的，目的：探讨异亚丙基莽草酸（3，4-oxo-isopropylidenshikimic acid，3，4-OIC）对2，4，6-三硝基苯磺酸（2，4，6-trinitrobenzenesulfonate，TNBS）诱导的大鼠实验性结肠炎模型的作用及其机制。对实验性结肠炎大鼠给予伊萨（50、100和200 mg/kg）12天。通过肉眼损伤评分、结肠重量/长度比（mg/cm）和髓过氧化物酶（MPO）活性评估炎症程度。结果：伊萨能明显改善结肠组织的大体损伤，降低结肠重量/长度比，降低MPO活性，降低MDA、NO水平和iNOS活性，提高GSH水平和SOD、GSH-Px活性，并呈剂量依赖性。此外，伊萨（200 mg/kg）的效果是有效的柳氮磺胺吡啶（500 mg/kg.Conclusions：这项研究的结果表明伊萨对实验性结肠炎的保护作用，可能是由于抗氧化作用。
Background:3,4-Oxo-isopropylidene-shikimic acid (ISA) is a derivative of shikimic acid (SA). SA is extracted from Illicium verum Hook.fil., which has been used in traditional Chinese medicine and used for treating vomiting, stomach aches, insomnia, skin inflammation, and rheumatic pain.Aims:To investigate the effects and the protective mechanism of 3,4-oxo-isopropylidene-shikimic acid on experimental colitis model induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) in rats.Methods:Colitis in rats was induced by colonic administration with TNBS. ISA (50, 100, and 200 mg/kg) was administered for 12 days to experimental colitis rats. The inflammatory degree was assessed by macroscopic damage score, colon weight/length ratios (mg/cm), and myeloperoxidase (MPO) activity. Malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) levels, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), inducible nitric oxide synthase (iNOS) activities were measured with biochemical methods.Results:ISA significantly ameliorated macroscopic damage, reduced colon weight/length ratios and the activity of MPO, depressed MDA and NO levels and iNOS activity, and enhanced GSH level, and GSH-Px and SOD activities in the colon tissues of experimental colitis in a dose-dependent manner. Moreover, the effect of ISA (200 mg/kg) was as effective as sulfasalazine (500 mg/kg).Conclusions:The findings of this study demonstrate the protective effect of ISA on experimental colitis, probably due to an antioxidant action.