INCREASED TYPE I COLLAGEN IN UNDAMAGED CARTILAGE OF ANTEROMEDIAL OSTEOARTHRITIS OF THE KNEE
INCREASED TYPE I COLLAGEN IN UNDAMAGED CARTILAGE OF ANTEROMEDIAL OSTEOARTHRITIS OF THE KNEE
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发表时间：
2009-09
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通讯作者：
S. McDonnell;R. Rout;A. Hollander;I. Clark;T. Simms;R. Davidson;S. Dickinson;J. Waters;H. Gill;D. Murray;P. Hulley;A. Price
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作者：
S. McDonnell;R. Rout;A. Hollander;I. Clark;T. Simms;R. Davidson;S. Dickinson;J. Waters;H. Gill;D. Murray;P. Hulley;A. Price
我们的目的是研究前内侧关节病 (AMG) 表型内软骨损伤逐渐严重的分子特征。从接受单间室膝关节置换术的患者身上采集了 10 份内侧胫骨平台标本。宏观损伤区域内的软骨被分成相等的三等份：T1（受损最严重），到T3（受损最轻）。宏观上未受损的软骨区域作为第四个样本，N。制备样本用于组织学（番红-O和H&E染色）和免疫组织化学分析（I型和II型胶原、增殖和凋亡）。对 I 型和 II 型胶原蛋白以及 GAG 含量进行免疫测定。实时 PCR 比较了 T 区和 N 区之间的基因表达。四个区域的 OARSI 等级均下降，T1、T2 和 T3 区之间的颤动逐渐减少。 N 区的 OARSI 等级为 0（正常）。 GAG 免疫测定显示，随着软骨损伤严重程度的增加，GAG 水平降低（p 在 AMG 中，存在明显的区域，表明进行性软骨损失。我们得出的结论是，在这种表型中，在宏观和组织学正常软骨区域中，I 型胶原蛋白增加，可能代表骨关节炎疾病过程中软骨基质的非常早期的变化。这可能能够用作早期疾病的测定以及作为疾病调整或治疗的治疗靶点。
Our aim was to investigate the molecular features of progressive severities of cartilage damage, within the phenotype of Anteromedial Gonarthrosis (AMG). Ten medial tibial plateau specimens were collected from patients undergoing unicompartmental knee replacements. The cartilage within the area of macroscopic damage was divided into equal thirds: T1(most damaged), to T3 (least damaged). The area of macroscopically undamaged cartilage was taken as a 4th sample, N. The specimens were prepared for histological (Safranin-O and H&E staining) and immunohistochemical analysis (Type I and II Collagen, proliferation and apoptosis). Immunoassays were undertaken for Collagens I and II and GAG content. Real time PCR compared gene expression between areas T and N. There was a decrease in OARSI grade across the four areas, with progressively less fibrillation between areas T1, T2 and T3. Area N had an OARSI grade of 0 (normal). The GAG immunoassay showed decreased levels with increasing severity of cartilage damage (p In AMG there are distinct areas, demonstrating progressive cartilage loss. We conclude that in this phenotype the Collagen I increase, in areas of macroscopically and histologically normal cartilage, may represent very early changes of the cartilage matrix within the osteoarthritic disease process. This may be able to be used as an assay of early disease and as a therapeutic target for disease modification or treatment.