HOMEOBOX GENES MSX-1 AND MSX-2 ARE ASSOCIATED WITH INDUCTION AND GROWTH OF SKIN APPENDAGES
HOMEOBOX GENES MSX-1 AND MSX-2 ARE ASSOCIATED WITH INDUCTION AND GROWTH OF SKIN APPENDAGES
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DOI：
10.1111/1523-1747.ep12606960
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发表时间：
1995-05-01
影响因子：
6.5
通讯作者：
CHUONG, CM
中科院分区：
文献类型：
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作者：
NOVEEN, A;JIANG, TX;CHUONG, CM
皮肤附属器的形态发生机制是皮肤发育和愈合的基础问题。为了研究皮肤附属器诱导和生长的分子机制，我们研究了两个同源异型盒基因Msx-1和Msx-2在鸡胚胎皮肤发育过程中的表达。ii）Msx-1和Msx-2都在生长的羽芽上皮中表达，但在芽间上皮中不表达; iii）尽管大部分重叠，但两种Msx基因的表达之间存在差异，Msx-1更多地向前部表达，而Msx-2更多地向远端羽芽表达; iv）不存在如对于Hox A-D簇的成员观察到的身体位置特异性表达模式; v）在羽囊中，Msx-1和Msx-2表达于领部和倒钩脊上皮，这两个区域的细胞持续增殖; vi）当毛喉素（一种腺苷酸环化酶的激活剂）抑制羽芽生长时，两种基因的表达均降低。这些结果表明Msx基因在注定成为皮肤附属物的上皮结构域中特异性表达;它在皮肤附件形态发生中的功能可能是双重的，首先是使上皮细胞有能力成为皮肤附件，其次是使上皮细胞保持其持续生长的潜力。
The mechanism involved in the morphogenesis of skin appendages is a fundamental issue underlying the development and healing of skin, To identify molecules involved in the induction and growth of skin appendages, we studied the expression of two homeobox genes, Msx-1 and Msx-2, during embryonic chicken skin development, We found that i) both Msx-1 and Msx-2 are early markers of epithelial placodes for skin appendages; ii) both Msx-1 and Msx-2 are expressed in the growing feather bud epithelia but not in the interbud epithelia; iii) although mostly overlapping, there are differences between the expression of the two Msx genes, Msx-1 being expressed more toward the anterior whereas Msx-2 is expressed more toward the distal feather bud; iv) there is no body-position-specific expression pattern as was observed for members of the Hox A-D clusters; v) in the feather follicle, Msx-1 and 2 are expressed in the collar and barb ridge epithelia, both regions of continuous cell proliferation; vi) when feather-bud growth was inhibited by forskolin, an activator of adenylyl cyclase, the expression of both genes was reduced, These results showed that Msx genes are specifically expressed in epithelial domains destined to become skin appendages; Its function in skin-appendage morphogenesis may be twofold, first in making epithelial cells competent to become skin appendages and, second, in making epithelial cells maintain their potential for continuous growth.