Contrasting Toxin Selectivity between the Marine Pufferfish Takifugu pardalis and the Freshwater Pufferfish Pao suvattii
Contrasting Toxin Selectivity between the Marine Pufferfish Takifugu pardalis and the Freshwater Pufferfish Pao suvattii
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DOI：
10.3390/toxins11080470
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发表时间：
2019-08-01
期刊：
影响因子：
4.2
通讯作者：
Arakawa, Osamu
中科院分区：
文献类型：
--
作者：
Gao, Wei;Kanahara, Yoko;Arakawa, Osamu
为阐明海水和淡水河豚毒素选择性的差异，采用河豚毒素（TTX）和麻痹性贝类毒素（PSP; decarbamoylsaxitoxin（dcSTX）或saxitoxin（STX））对人工饲养的无毒性的太平洋东方鲀（Takifugu pardalis）（海水）和苏氏泡鲀（Pao suvattii）（淡水）进行了实验。T.通过经口管饲法，向豹斑鱼样本施用含有TTX或dcSTX（毒素剂量，55.2nmol/鱼）的饲料匀浆，并向苏瓦氏原螯虾样本施用含有TTX + STX（每种毒素剂量，19.2nmol/鱼）的饲料匀浆。在24和48或72 h后定量肠、肌肉、皮肤、肝脏和性腺中的毒素含量。于T.在pardalis中，施用到肠中的TTX被吸收到体内并主要转移和保留在皮肤和肝脏中，而dcSTX几乎不保留在体内，尽管它部分保留在肠中。与此形成强烈对比的是，在苏瓦氏疟原虫中，几乎没有TTX残留在体内，而STX被吸收到体内并转移和保留在卵巢和皮肤中。结果表明，不同海洋物种的T. pardalis和淡水物种P. suvattii。T.天然含有TTX的Pardalis选择性地积累TTX，而天然含有PSP的P. suvattii选择性地积累PSP。
To clarify the differences in toxin selectivity between marine and freshwater pufferfish, we conducted experiments in artificially reared nontoxic specimens of Takifugu pardalis (marine) and Pao suvattii (freshwater) using tetrodotoxin (TTX) and paralytic shellfish poison (PSP; decarbamoylsaxitoxin (dcSTX) or saxitoxin (STX)). T. pardalis specimens were administered feed homogenate containing TTX or dcSTX (dose of toxin, 55.2 nmol/fish) and P. suvattii specimens were administered feed homogenate containing TTX + STX (dose of each toxin, 19.2 nmol/fish) by oral gavage. The toxin content in the intestine, muscle, skin, liver, and gonads was quantified after 24 and 48 or 72 h. In T. pardalis, TTX administered into the intestine was absorbed into the body and transferred and retained mainly in the skin and liver, while dcSTX was hardly retained in the body, although it partly remained in the intestine. In strong contrast, in P. suvattii, little TTX remained in the body, whereas STX was absorbed into the body and was transferred and retained in the ovary and skin. The findings revealed that TTX/PSP selectivity differs between the marine species T. pardalis and the freshwater species P. suvattii. T. pardalis, which naturally harbors TTX, selectively accumulates TTX, and P. suvattii, which naturally harbors PSP, selectively accumulates PSP.