Extraction Separation of BSA in Aqueous Two-Phase Systems of Anionic and Cationic Surfactant Mixtures
Extraction Separation of BSA in Aqueous Two-Phase Systems of Anionic and Cationic Surfactant Mixtures
复制标题
DOI：
10.1080/01932691.2010.488141
复制
发表时间：
2011-05
影响因子：
2.2
通讯作者：
H. Teng;Ning Li;Xixi Zhu;Yong Chen
中科院分区：
文献类型：
--
作者：
H. Teng;Ning Li;Xixi Zhu;Yong Chen
双水相体系(ATP)在十二烷基硫酸钠(十二烷基硫酸钠)、十六烷基三甲基溴化铵(CTAB)和溴化钠(NaBR)的混合物中形成。当表面活性剂CTAB和十二烷基硫酸钠过量时，分别出现两种不同类型的ATP。这种ATPS可能为牛血清白蛋白(BSA)的分离提供一种新的、有用的分配体系。研究了牛血清白蛋白在这些体系中的分配。结果表明，ATPS顶相的电学性质、疏水性和液晶结构对其分配有影响。当LC和BSA带电相反时，BSA被提取到顶相，分配系数较高。液晶的疏水性可以通过增加烷基长度来提高，从而提高了分配系数。层状LC和六方LC的疏水性强于立方LC，导致前者的萃取能力高于后者。
Aqueous two-phase systems (ATPS) are formed in mixtures of sodium dodecylsulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), and sodium bromide (NaBr). Two different kinds of ATPS appear when CTAB and SDS surfactants are in excess, respectively. Such ATPS may provide a new, useful partitioning system for separation of bovine serum albumin (BSA). The partitioning of BSA was studied in these systems. The results show that the partitioning is affected by the electrical property, the hydrophobicity and the structure of liquid crystal (LC), which exists in the top phase of the ATPS. BSA is extracted into the top phase with higher distribution coefficient when LC and BSA are oppositely charged. The hydrophobicity of LC, which can be improved by increasing the length of alkyl group, enhances the distribution coefficient. The hydrophobicity of lamellar LC and hexagonal LC is stronger than that of cubic LC, which causes extractive capability of the former is higher than the latter.