Functional genomics: High-throughput mRNA, protein, and metabolite analyses
Functional genomics: High-throughput mRNA, protein, and metabolite analyses
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DOI：
10.1006/mben.2001.0212
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发表时间：
2002-01-01
影响因子：
8.4
通讯作者：
Wurtele, ES
中科院分区：
文献类型：
--
作者：
Oliver, DJ;Nikolau, B;Wurtele, ES
现在科学家和工程师可以获得大量的DNA序列信息。这些DNA序列为研究生物体的基因组如何运作提供了基础，它们对对操纵植物生产化学物质和酶感兴趣的代谢工程师特别有用。功能基因组学依靠高通量技术来测量mRNA（转录组）和蛋白质（蛋白质组）。以及植物及其器官和组织的代谢物（代谢组）成分。微阵列技术、蛋白质质谱分析和高通量代谢物分析的最新进展开始提供有关植物总mRNA、蛋白质和代谢物成分的详细信息。这些知识将使科学家能够监测植物中蛋白质和代谢物的变化。最终，它可能使他们发现新的代谢途径，并模拟植物的代谢和调节网络。(C) 2002 Elsevier Science。
A tremendous amount of DNA sequence information is now available to scientists and engineers. These DNA sequences provide the foundation for studying how the genome of an organism is functioning and they are particularly useful for metabolic engineers interested in manipulating plants for the production of chemicals and enzymes. Functional genomics relies on high-throughput techniques for measuring the mRNA (the transcriptome), protein (the proteome). and metabolite (the metabolome) components of plants as well as their organs and tissues. Microarray technologies, recent advances in protein mass spectrometry, and high-throughput metabolite analyses are beginning to provide detailed information on the total mRNA, protein, and metabolite components of plants. This knowledge will allow scientists to monitor changes in proteins and metabolites in plants. Ultimately, it may allow them to discover new metabolic pathways and to model metabolic and regulatory networks in plants. (C) 2002 Elsevier Science.