Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
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DOI：
10.1007/978-1-61779-468-1_6
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
Banas, Agnieszka
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作者：
Banas, Agnieszka
从不同类型的干细胞体外培养成有功能的肝细胞一直是人们研究的热点。多潜能间充质干细胞(MSC)构成了干细胞治疗的重要来源，尤其是因为它们可以从患者自己的组织中获得，避免了免疫兼容性和伦理问题。在不同来源的MSCs中，脂肪组织来源的间充质干细胞(AT-MSCs)因其高可获得性、增殖能力、潜能和免疫相容性而被认为是非常有前途的干细胞。它们代表了一个不同种类的细胞群体。AT-MSCs的确切标记物尚未确定，因此，如何纯化这些异质性细胞仍不是很明显。我们推测定义MSC来源的标志物之一是CD105(Endoglin)。因此，我们对AT-MSCs的CD105(+)部分进行了分选，对其进行扩增，并向肝样细胞分化。为了考察CD105(+)AT-MSCs向中胚层分化的潜能，我们首先用商业化的方法将CD105(+)AT-MSCs分化为中胚层细胞。我们发现，纯净的CD105(+)AT-MSCs部分显示出更高的同质性和向成脂、成骨、成软骨的分化潜能，并高度诱导成肝细胞系。(使用我们的肝脏分化方案)CD105(+)AT-MSCs来源的肝样细胞表达肝细胞标志物、酶和功能。
There is a great interest in the development of functional hepatocytes in vitro from different types of stem cells. Multipotential mesenchymal stem cells (MSC) compose a great source for stem cell based therapy, especially, because they can be obtain from patients own tissues, sidestepping immunocompatibility and ethical issues. Among MSCs from different sources, adipose-tissue-derived mesenchymal stem cells (AT-MSCs) are very promising because of their high accessibility, proliferation ability, potentiality, and immunocompatibility.AT-MSCs can be easily isolated from stroma vascular fraction (SVF) of adipose tissue. They represent a heterogeneous population of cells. The precise AT-MSCs's marker profile has not been defined yet; therefore, it is still not obvious how to purify these heterogeneous fraction of cells. We postulate that one of the markers defining MSC provenance is CD105 (endoglin).Therefore, we have sorted CD105(+) fraction of AT-MSCs, expanded them, and differentiated toward hepatic-like cells. In order to check their potentiality, we have firstly differentiated sorted CD105(+) AT-MSCs toward mesoderm lineages, using commercialized protocols.We have shown here, that pure CD105(+) AT-MSCs fraction revealed higher homogeneity and differentiation potential toward adipogenic, osteogenic, and chondrogenic lineages and highly inducible into the hepatogenic lineage.Generated (by using our hepatic differentiation protocol) CD105(+) AT-MSCs-derived hepatic-like cells expressed hepatocyte markers, enzymes, and functions.