Long-circulating near-infrared fluorescence core-cross-linked polymeric micelles: Synthesis, characterization, and dual nuclear/optical imaging
Long-circulating near-infrared fluorescence core-cross-linked polymeric micelles: Synthesis, characterization, and dual nuclear/optical imaging
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DOI：
10.1021/bm7005399
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发表时间：
2007-11-01
影响因子：
6.2
通讯作者：
Li, Chun
中科院分区：
文献类型：
--
作者：
Yang, Zhi;Zheng, Shiying;Li, Chun
我们报道了由胺封端的两亲性嵌段共聚物聚（PEG-甲基丙烯酸酯）-b-聚（甲基丙烯酸三乙氧基甲硅烷基丙酯）衍生的 PEG 涂层、核交联聚合物胶束（CCPM）的合成。嵌段共聚物自组装形成胶束纳米颗粒，并且通过随后的溶胶-凝胶过程，类 Cy-7 近红外荧光 (NIRF) 染料被捕获在带有反应性乙氧基硅烷官能团的核心中。 CCPM 的摩尔荧光信号比 Cy7 亮 16 倍。 CCPM 的平均直径为 24 +/- 8.9 nm，具有较长的血液半衰期（t(1/2,alpha) = 1.25 h；t1/2,beta = 46.18 h），并且被单核吞噬系统中等程度摄取。 CCPM 在人类乳腺肿瘤异种移植物中的显着积累允许使用 NIRF 光学和伽马成像技术对摄取动力学进行无创监测。我们的数据表明，Cy7 包埋的 CCPM 纳米粒子适用于实体瘤的 NIRF 成像，并且在肿瘤相关分子标记物的成像中具有潜在的应用。
We report the synthesis of PEG-coated, core-cross- linked polymeric micelles (CCPMs) derived from an amine-terminated amphiphilic block copolymer, poly(PEG-methacrylate)-b-poly(triethoxysilyl propylmethacrylate). The block copolymer self-assembled to form micellar nanoparticles, and a Cy-7-like near-infrared fluorescence (NIRF) dye was entrapped in the core bearing reactive ethoxysilane functional groups through a subsequent sol-gel process. The fluorescent signal of CCPMs on the molar basis was 16-fold brighter than that of Cy7. With an average diameter of 24 +/- 8.9 nm, CCPMs exhibited a prolonged blood half-life (t(1/2,alpha) = 1.25 h; t1/2,beta = 46.18 h) and moderate uptake by the mononuclear phagocytic system. Significant accumulation of CCPMs in human breast tumor xenografts allowed noninvasive monitoring of the uptake kinetics with both NIRF optical and gamma imaging techniques. Our data suggest that Cy7-entrapped CCPM nanoparticles are suitable for NIRF imaging of solid tumors and have potential applications in the imaging of tumor-associated molecular markers.