A Pod Leakage Technique for Phloem Translocation Studies in Soybean (Glycine max [L.] Merr.).
A Pod Leakage Technique for Phloem Translocation Studies in Soybean (Glycine max [L.] Merr.).
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用于大豆韧皮部易位研究的豆荚泄漏技术（Glycine max [L.] Merr.）。
DOI：
10.1104/pp.62.5.812
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发表时间：
1978
期刊：
影响因子：
7.4
通讯作者：
J. Leggett
中科院分区：
文献类型：
--
作者：
R. J. Fellows;D. Egli;J. Leggett
将放射性光合同化物转移到大豆（Glycine max [L.] Merr. Fiskeby V ′）荚的花柱顶端可以通过在20毫米乙二胺四乙酸盐溶液（pH 7.0）下切下荚的花柱顶端并使材料渗漏到溶液中来直接测量。源节点处的吊舱接收从源叶输出到吊舱的（14）C的大约50%，并将其中的大约1%至3%泄漏到溶液中。超过90%的（14）C从豆荚泄漏被发现在中性馏分，其中，约93%是在蔗糖。在泄漏中鉴定了15种氨基酸，包括：丙氨酸、精氨酸、天冬酰胺、γ-氨基丁酸、谷氨酰胺、甘氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、苯丙氨酸、丝氨酸、苏氨酸、酪氨酸和缬氨酸。碱性组分中的大部分（14）C存在于丝氨酸（约30%）和天冬酰胺（约23%）中。无机离子K、Ca、P、Mg、Zn和Fe在渗漏组分中被发现。在收集的泄漏溶液中未检测到硝酸盐。NO（3）（-）的缺乏和蔗糖中标记物的大比例表明大多数材料可能起源于韧皮部。该技术提供了一个简单的，可重复的手段，分析的材料转移到和通过大豆豆荚，以及以下的标签到达豆荚的时间过程。
Radioactive photosynthetic assimilates, translocated to a soybean (Glycine max [L.] Merr. ;Fiskeby V') pod can be measured directly by excising the stylar tip of the pod under 20 mm ethylenediaminetetraacetate solution (pH 7.0) and allowing the material to leak into the solution. Pods at the source node received approximately 50% of the (14)C exported from the source leaf to the pod and leaked approximately 1 to 3% of this into the solution. More than 90% of the (14)C that leaked from the pods was found in the neutral fraction and, of this, about 93% was in sucrose. Fifteen amino acids were identified in the leakage including: alanine, arginine, asparagine, gamma-aminobutyric acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, phenylalanine, serine, threonine, tyrosine, and valine. The majority of the (14)C in the basic fraction was found in serine ( approximately 30%) and asparagine ( approximately 23%). The inorganic ions K, Ca, P, Mg, Zn, and Fe were found in the leakage component. Nitrate was not detectable in the collected leakage solution. The absence of NO(3) (-) and the large proportion of the label in sucrose suggest a possible phloem origin for most of the material. The technique provides an uncomplicated, reproducible means of analyzing the material translocated into and through the soybean pod, as well as following the time course of label arrival at the pod.