Disassembly-Driven Fluorescence Turn-on of Polymerized Micelles by Reductive Stimuli in Living Cells
Disassembly-Driven Fluorescence Turn-on of Polymerized Micelles by Reductive Stimuli in Living Cells
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DOI：
10.1002/chem.201405040
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发表时间：
2014-12-08
影响因子：
4.3
通讯作者：
Klymchenko, Andrey S.
中科院分区：
文献类型：
--
作者：
Niko, Yosuke;Arntz, Youri;Klymchenko, Andrey S.
刺激反应纳米颗粒已经成为成像和治疗应用的强大工具。理想情况下，它们应该由可生物降解的材料组装而成，这些材料具有小尺寸和对生物刺激的合作反应，生物刺激触发颗粒分解和活性分子的释放，可以很容易地进行现场监测。发展了一种概念，它由有机纳米颗粒组成，由荧光两亲分子组装，并与可氧化还原裂解的交联剂聚合。我们得到了含有自猝灭尼罗红染料残留物的20纳米纳米颗粒，在外部或内部还原刺激下，它们可以在活细胞中分解成高荧光的分子单元。所获得的结果为新的刺激响应纳米材料在无背景成像以及药物输送中的应用铺平了道路，因为这一概念可以进一步扩展到包括药物在内的其他活性分子和可被其他生物刺激切割的交联剂。
Stimuli-response nanoparticles have emerged as powerful tools for imaging and therapeutic applications. Ideally, they should be assembled from biodegradable materials featuring small size and cooperative response to biological stimuli that trigger particle disassembly and release of an active molecule that could be readily monitored in situ. A concept is developed that consists of organic nanoparticles, assembled from fluorescent amphiphiles and polymerized with a redox-cleavable cross-linker. We obtained 20 nm nanoparticles bearing self-quenched Nile Red dye residues, which can disassemble in living cells into highly fluorescent molecular units owing to an external or internal reductive stimulus. The obtained results pave the way to new stimuli-responsive nanomaterials for applications in background-free imaging as well as in drug delivery, as the concept can be further extended to other active molecules including drugs and to crosslinkers cleavable by other biological stimuli.