Phosphomonoester concentrations differ between chronic lymphocytic leukemia cells and normal human lymphocytes
Phosphomonoester concentrations differ between chronic lymphocytic leukemia cells and normal human lymphocytes
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DOI：
10.1016/s0145-2126(02)00035-8
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发表时间：
2002-10-01
影响因子：
2.7
通讯作者：
Brown, TR
中科院分区：
文献类型：
--
作者：
Franks, SE;Smith, MR;Brown, TR
采用磷磁共振波谱法定量测定慢性淋巴细胞白血病淋巴细胞（CLL）和正常人淋巴细胞的磷脂相关代谢物水平。CLL细胞相对于正常淋巴细胞显示磷酸乙醇胺（Etn-P）的显著增加（8.11 +/-2.10平均值+/- S.E.，μ mol/g湿重，n = 12对3.63 +/- 1.10，n = 3，P < 0.002），磷酸胆碱（2.10 +/- 0.37，n = 12对比0.36 +/- 0.09，n = 3，P ≤ 0.01），甘油磷酸乙醇胺（0.26 +/- 0.03，n = 10对比0.11 +/- 0.05，n = 3 P小于或等于0.004）。和甘油磷酸胆碱（0.33 +/-0.03，n = 10对0. 17 +/- 0.05，n = 3，P小于或等于0.003）。此外，研究了血液中的磷脂前体乙醇胺（Eth），发现其在CLL患者中显著降低（4.6 +/-16 μ M）。n = 25）与正常志愿者（7.7 +/- 2.5，n = 12，P小于或等于0.001）相比。增加的中间体，与耗尽的前体表明存在持续的磷脂代谢活化的CLL。(C)2002年由Elsevier Science Ltd.出版
Levels of phospholipid-related metabolites of chronic lyniphocytic leukemia lymphocytes (CLL) and normal human lymphocytes were quantified using phosphorus magnetic resonance spectroscopy. The CLL cells versus normal lymphocytes showed significant increases of phosphoethanolamine(Etn-P) (8.11 +/- 2.10 mean +/- S.E., mumol/g wet weight, n = 12 versus 3.63 +/- 1.10, n = 3, P < 0.002), phosphocholine (2.10 +/- 0.37, n = 12 versus 0.36 +/- 0.09, n = 3, P less than or equal to 0.01), glycerophosphoethanolarnine (0.26 +/- 0.03, n = 10 versus 0.11 +/- 0.05, n = 3 P less than or equal to 0.004). and glycerophosphocholine (0.33 +/- 0.03, n = 10 versus 0. 17 +/- 0.05, n = 3, P less than or equal to 0.003). Further, the phospholipid precursor ethanolamine (Eth) was studied in blood and was found significantly lowered in CLL patients (4.6 +/- 1 6 muM. n = 25) compared to normal Volunteers (7.7 +/- 2.5, n = 12, P less than or equal to 0.001). Increased intermediate,, with depletion of precursors suggest the presence of sustained phospholipid metabolism activation in CLL. (C) 2002 Published by Elsevier Science Ltd.