Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy
Circular RNA expression in isoproterenol hydrochloride-induced cardiac hypertrophy
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盐酸异丙肾上腺素诱导的心脏肥大中环状RNA的表达
DOI：
10.18632/aging.102761
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发表时间：
2020
期刊：
影响因子：
5.2
通讯作者：
Xue Li-Xiang
中科院分区：
文献类型：
--
作者：
Yang Ming-Hui;Wang Hao;Han Sheng-Na;Jia Xin;Zhang Si;Dai Fei-Fei;Zhou Meng-Jiao;Yin Zhongnan;Wang Tian-Qi;Zang Ming-Xi;Xue Li-Xiang
环状RNA（circular RNA，circRNA）是一类新的非编码RNA，其在心肌肥厚发生发展中的作用尚待进一步研究。在这里，我们研究了circRNA在心脏肥大中的潜在作用。通过对8周龄盐酸异丙肾上腺素诱导的心肌肥厚小鼠的左心室标本进行circRNA测序，我们发现与对照组相比，在3323个总circRNA中，有401个在肥厚心脏中失调。其中，303个circRNA上调，98个下调。GO和KEGG分析表明，大部分差异表达circRNA的亲本基因不仅与代谢过程、刺激反应等生物学过程相关，还与循环系统、心血管疾病等通路相关。另一方面，预测了1974个miRNAs与这些差异表达的circRNAs结合，并对这些miRNAs可能的靶mRNA进行了功能注释和分析。最后，我们确定了ANF和miR-23 a是circRNA wwp 1的下游靶点，表明circRNA wwp 1通过下调ANF和miR-23 a发挥抑制心肌肥大的作用，这是circRNA调节心肌肥大的潜在机制。
Circular RNA (circRNA) is a novel class of noncoding RNAs, and the roles of circRNAs in the development of cardiac hypertrophy remain to be explored. Here, we investigate the potential roles of circRNAs in cardiac hypertrophy. By circRNA sequencing in left ventricular specimens collected from 8-week-old mice with isoproterenol hydrochloride-induced cardiac hypertrophy, we found 401 out of 3323 total circRNAs were dysregulated in the hypertrophic hearts compared with the controls. Of these, 303 circRNAs were upregulated and 98 were downregulated. Moreover, the GO and KEGG analyses revealed that the majority of parental gene of differentially expressed circRNAs were not only related to biological process such as metabolic process and response to stimulus, but also related to pathway such as circulatory system and cardiovascular diseases. On the other hand, total 1974 miRNAs were predicted to binding to these differentially expressed circRNAs, and the possible target mRNAs of those miRNAs were also predicted and analyzed in terms of functional annotation. Finally, we identified that ANF and miR-23a are downstream targets of circRNA wwp1, suggesting that circRNA wwp1 exerts inhibitory roles of cardiac hypertrophy via down-regulation of ANF and miR-23a, which underlying the potential mechanisms whereby circRNA regulates cardiac hypertrophy.