Facile degradation of apolipoprotein B by radical reactions and the presence of cleaved proteins in serum
Facile degradation of apolipoprotein B by radical reactions and the presence of cleaved proteins in serum
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DOI：
10.1093/oxfordjournals.jbchem.a022256
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发表时间：
1999-01-01
影响因子：
2.7
通讯作者：
Kojo, S
中科院分区：
文献类型：
--
作者：
Tanaka, K;Iguchi, H;Kojo, S
本文报道载脂蛋白B（apoB）的自由基裂解反应。用Cu ~（2+）氧化损伤人低密度脂蛋白（LDL），免疫印迹法观察到apoB的降解。自由基清除剂可抑制apoB的降解（β-巯基乙醇、丁基化羟基甲苯和普罗布考）并由自由基引发剂[2，2 '-偶氮二当人血清用Cu ~（2+）处理时，观察到类似的apoB切割模式。这些结果表明，apoB在体外和体内对自由基具有高度反应性，反应导致肽键的断裂。
A facile cleavage of peptide bonds of apolipoprotein B (apoB) by radical reaction is reported, When human LDL was subjected to oxidative damage using Cu2+, extensive degradation of apoB was observed based on immunoblotting, The degradation of apoB was inhibited by radical scavengers (beta-mercaptoethanol, butylated hydroxytoluene, and probucol) and promoted by a radical initiator [2,2'-azobis(2-amidinopropane)dihydrochloride], When human serum was treated with Cu2+, a similar cleavage pattern of apoB was observed. The cleaved apoB proteins were also detected in normal serum on the basis of immunoblots, These results suggest that apoB is highly reactive toward radicals in vitro and in vivo, with reaction resulting in the cleavage of peptide bonds.