THE EFFECTS OF HEXACHLOROBENZENE ON CIRCULATING LEVELS OF ADRENAL-STEROIDS IN THE OVARIECTOMIZED RAT
THE EFFECTS OF HEXACHLOROBENZENE ON CIRCULATING LEVELS OF ADRENAL-STEROIDS IN THE OVARIECTOMIZED RAT
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DOI：
10.1002/jbt.2570100303
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发表时间：
1995-06-01
期刊：
影响因子：
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通讯作者：
LECAVALIER, P
中科院分区：
文献类型：
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作者：
FOSTER, WG;MERTINEIT, C;LECAVALIER, P
六氯苯（HCB）是一种全球性污染物，不易降解，并具有生物累积的倾向。然而，六氯苯对肾上腺功能的影响在很大程度上仍然未知。因此，对接触六氯苯（0.0、1.0、10.0或100.0毫克/千克/天-连续灌胃30天）的切除卵巢的成年Sprague-Dawley大鼠（N = 32）体内肾上腺类固醇的循环水平进行了研究。收集末梢血液样本进行六氯苯残留分析，并对循环孕酮（P-4）、皮质酮（CS）和醛固酮（ALD）水平进行定量。从最低剂量组（1.0 mg/kg/天，持续30天）开始，六氯苯暴露显著降低了平均血清CS水平（P = 0.02），而观察到平均血清P-4和ALD水平无差异。由于啮齿类动物具有产生少量皮质醇的能力，并且这种糖皮质激素的水平在病理状态下会改变，因此还测量了血清皮质醇（C）水平。与对照组相比，最高剂量组的循环C水平显著降低（P < 0.05）。通过反相HPLC确认血清中C的存在。这些数据表明，即使在研究的最低剂量下，六氯苯暴露也会引起肾上腺皮质内区细胞类固醇生成的改变。
Hexachlorobenzene (HCB), is a global pollutant that resists degradation and possesses a propensity to bioaccumulate. However, the effect of HCB on adrenal function remains largely unknown. Thus, circulating levels of adrenal steroids in HCB-exposed (0.0, 1.0, 10.0, or 100.0 mg/kg/day-for 30 days by gavage) adult ovariectomized Sprague-Dawley rats (N = 32) were investigated. A terminal blood sample was collected for HCB residue analysis, and levels of circulating progesterone (P-4), corticosterone (CS), and aldosterone (ALD) were quantified. Mean serum CS levels were significantly (P = 0.02) reduced by HCB exposure, starting with the lowest dose group (1.0 mg/kg/day for 30 days), whereas no differences in mean serum P-4 and ALD levels were observed. Since it has been argued that the rodent possesses the ability to produce small amounts of cortisol and that levels of this glucocorticoid are altered in pathological states, serum cortisol (C) levels were also measured. Circulating levels of C were significantly lower (P < 0.05) in the highest dose group compared with controls. The presence of C in serum was confirmed by reverse-phase HPLC. These data suggest that even at the lowest dose studied, HCB exposure induces alterations in steroidogenesis of cells of the adrenal cortex inner zone.