DEVELOPMENTAL POTENTIAL OF THE EARLIEST PRECURSOR CELLS FROM THE ADULT-MOUSE THYMUS
DEVELOPMENTAL POTENTIAL OF THE EARLIEST PRECURSOR CELLS FROM THE ADULT-MOUSE THYMUS
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DOI：
10.1084/jem.174.6.1617
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发表时间：
1991-12-01
影响因子：
15.3
通讯作者：
SHORTMAN, K
中科院分区：
文献类型：
--
作者：
WU, L;ANTICA, M;SHORTMAN, K
最近分离出一种新的，数值上微小的细胞群，代表成年小鼠胸腺中最早的T前体细胞。该群体已被证明在表面抗原表型方面与骨髓造血干细胞相似，并表达中等水平的CD 4。我们现在通过荧光激活细胞分选和胸腺内转移到受辐射的小鼠，表明这种明显同质的群体在表达表面干细胞抗原2（Sca-2）方面不同于多能干细胞，在缺乏B220和II类主要组织相容性复合物表达方面不同于大多数早期B谱系细胞，并且它与罗丹明123的结合类似于活化细胞而不是静止细胞。辐照受体小鼠不同的赖氨酸5位点被用来比较这些早期胸腺内前体与骨髓干细胞的发育潜力。当胸腺内前体细胞群被转移回辐照胸腺时，仅检测到T谱系产物细胞。然而，当胸腺内前体细胞群被静脉内转移时，它显示出在受体骨髓、脾脏和淋巴结中发育成B和T淋巴细胞的能力，但未检测到供体来源的髓样细胞。通过显示胸腺内前体群体在静脉转移时无法发育成骨髓或红细胞脾集落或在琼脂培养物中形成集落，证实了不存在骨髓和红细胞前体活性。这些发现表明，这种最早的胸腺内前体细胞群已成为限制（或强烈偏见）淋巴谱系的发展，但不完全是T淋巴细胞。
A new, numerically minute population of cells representing the earliest T precursor cells in the adult mouse thymus has recently been isolated. This population has been shown to be similar to bone marrow hemopoietic stem cells in surface antigenic phenotype and to express moderate levels of CD4. We now show, by fluorescence-activated cell sorting and intrathymic transfer to irradiated mice, that this apparently homogeneous population differs from multipotent stem cells in expressing the surface stem cell antigen 2 (Sca-2), that it differs from most early B lineage cells in lacking B220 and class II major histocompatibility complex expression, and that it binds rhodamine 123 like an activated rather than a quiescent cell. Irradiated recipient mice differing at the Ly 5 locus were used to compare the developmental potential of these early intrathymic precursors with bone marrow stem cells. Only T lineage product cells were detected when the intrathymic precursor population was transferred back into an irradiated thymus. However, when the intrathymic precursor population was transferred intravenously, it displayed the capacity to develop into both B and T lymphoid cells in recipient bone marrow, spleen, and lymph nodes, but no donor-derived myeloid cells were detected. The absence of myeloid and erythroid precursor activity was confirmed by showing that the intrathymic precursor population was unable to develop into myeloid or erythroid spleen colonies on intravenous transfer or to form colonies in an agar culture. These findings indicate that this earliest intrathymic precursor population has become restricted (or strongly biased) to lymphoid lineage development, but not exclusively to T lymphocytes.