Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family
Cloning of two novel human importin-alpha subunits and analysis of the expression pattern of the importin-alpha protein family
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DOI：
10.1016/s0014-5793(97)01265-9
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发表时间：
1997-11-03
期刊：
影响因子：
3.5
通讯作者：
Hartmann, E
中科院分区：
文献类型：
--
作者：
Kohler, M;Ansieau, S;Hartmann, E
许多蛋白质进入细胞核是由importin-alpha/beta异源二聚体介导的。虽然只发现了一个importin-beta基因，但已经描述了几种形式的importin-alpha。除了已经确定的三种人类输入素α外，我们在这里报告了两种新的人类输入素α蛋白的一级结构。五个已知的人类输入素α亚基可以分为三个亚家族，在高等真核生物中似乎是保守的。我们通过免疫印迹表明，不同的重要蛋白-α亚家族在多种人体组织和哺乳动物细胞系中表达。(C)1997年欧洲生物化学学会联合会。
The import of many proteins into the nucleus is mediated by the importin-alpha/beta heterodimer. While only one importin-beta gene has been found, several forms of importin-alpha have been described. In addition to the three human importin-alpha s already identified, we report here the primary structure of two new human importin-alpha proteins. The five known human importin-alpha subunits can be classified into three subfamilies that appear conserved in higher eukaryotic organisms. We show by immunoblotting that the different importin-alpha subfamilies are expressed in a variety of human tissues and mammalian cell lines.(C) 1997 Federation of European Biochemical Societies.