In situ forming poly(ethylene glycol)-based hydrogels via thiol-maleimide Michael-type addition.
In situ forming poly(ethylene glycol)-based hydrogels via thiol-maleimide Michael-type addition.
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DOI：
10.1002/jbm.a.33106
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Kao WJ
中科院分区：
文献类型：
--
作者：
Fu Y;Kao WJ
细胞和敏感化合物的掺入可以在不存在UV或在生物医学水凝胶如聚（乙二醇）水凝胶的形成中常见的其他能量源的情况下更好地促进。通过逐步增长聚合的马来酰亚胺和硫醇封端的聚（乙二醇）大分子单体通过迈克尔型加成的水凝胶的形成进行了描述。研究了大分子单体浓度、pH值、温度和生物分子明胶对凝胶形成的影响。马来酰亚胺和硫醇官能团之间的反应动力学被发现是快速的。分子量随时间的增加，其特征在于通过凝胶渗透色谱法在逐步增长聚合。溶胀和降解结果表明，加入明胶增强溶胀和加速降解。增加明胶含量导致储能模量（G '）降低。FITC标记的葡聚糖从所得基质中的体外释放动力学表明，在新的原位凝胶形成药物递送系统的发展潜力。此外，所得网络对原代人单核细胞、成纤维细胞和角质形成细胞的粘附性最低，从而为进一步生物功能化以指导特异性生物反应提供了理想的平台。
The incorporation of cells and sensitive compounds can be better facilitated without the presence of UV or other energy sources that are common in the formation of biomedical hydrogels such as poly(ethylene glycol) hydrogels. The formation of hydrogels by the step-growth polymerization of maleimide- and thiol-terminated poly(ethylene glycol) macromers via Michael-type addition is described. The effects of macromer concentration, pH, temperature, and the presence of biomolecule gelatin on gel formation were investigated. Reaction kinetics between maleimide and thiol functional groups were found to be rapid. Molecular weight increase over time was characterized via gel permeation chromatography during step-growth polymerization. Swelling and degradation results showed incorporating gelatin enhanced swelling and accelerated degradation. Increasing gelatin content resulted in the decreased storage modulus (G’). The in vitro release kinetics of FITC-labeled dextran from the resulting matrices demonstrated the potential in the development of novel in situ gel-forming drug delivery systems. Moreover, the resulting networks were minimally adhesive to primary human monocytes, fibroblasts and keratinocytes thus providing an ideal platform for further biofunctionalizations to direct specific biological response.