Variation in leaf transcriptome responses to elevated ozone corresponds with physiological sensitivity to ozone across maize inbred lines.
Variation in leaf transcriptome responses to elevated ozone corresponds with physiological sensitivity to ozone across maize inbred lines.
复制标题
叶子转录组对臭氧升高的反应的变化与玉米自交系对臭氧的生理敏感性相对应。
DOI：
10.1093/genetics/iyac080
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发表时间：
2022
期刊：
影响因子：
3.3
通讯作者：
McIntyre,LaurenM
中科院分区：
文献类型：
--
作者：
Nanni,AdalenaV;Morse,AlisonM;Newman,JeremyRB;Choquette,NicoleE;Wedow,JessicaM;Liu,Zihao;Leakey,AndrewDB;Conesa,Ana;Ainsworth,ElizabethA;McIntyre,LaurenM
我们研究了持续升高的臭氧浓度对5种不同玉米自交系基因型叶片转录组的影响，这些基因型对臭氧的生理敏感性不同（B73，Mo 17，Hp 301，C123和NC 338），使用长读段组装转录本和短读段量化这些转录本的表达。超过99%的长读段、99%的组装转录物和97%的短读段映射到B73和Mo 17参考基因组。约95%的基因与组装的转录本属于已知的B73-Mo 17同线位点和94%的基因与组装的转录本存在于所有温带系的巢式关联作图泛基因组。虽然有有限的证据选择性剪接在臭氧胁迫的反应，有5种基因型之间的差异表达的幅度的差异。臭氧抗性B73基因型（151个基因）对持续臭氧胁迫的转录反应是适度的，而在更敏感的NC 338基因型中有超过3，300个基因显著差异表达。30%的基因有串联重复的可能，但潜在的串联重复与差异表达之间没有明显的联系。在5种基因型（83个基因）中具有共同响应的基因与光合作用相关，特别是光系统I。在B73中不差异表达但在其他4种基因型中响应的基因的功能注释（789）鉴定了活性氧物质。这表明B73对长期臭氧暴露的反应与其他4种基因型不同。基因型对臭氧的响应的相对大小和富集分析是一致的，无论短读段是否与长读段组装的转录物、B73参考、Mo 17参考比对。我们发现，长时间的臭氧暴露直接影响叶片的光合机制。
We examine the impact of sustained elevated ozone concentration on the leaf transcriptome of 5 diverse maize inbred genotypes, which vary in physiological sensitivity to ozone (B73, Mo17, Hp301, C123, and NC338), using long reads to assemble transcripts and short reads to quantify expression of these transcripts. More than 99% of the long reads, 99% of the assembled transcripts, and 97% of the short reads map to both B73 and Mo17 reference genomes. Approximately 95% of the genes with assembled transcripts belong to known B73–Mo17 syntenic loci and 94% of genes with assembled transcripts are present in all temperate lines in the nested association mapping pan-genome. While there is limited evidence for alternative splicing in response to ozone stress, there is a difference in the magnitude of differential expression among the 5 genotypes. The transcriptional response to sustained ozone stress in the ozone resistant B73 genotype (151 genes) was modest, while more than 3,300 genes were significantly differentially expressed in the more sensitive NC338 genotype. There is the potential for tandem duplication in 30% of genes with assembled transcripts, but there is no obvious association between potential tandem duplication and differential expression. Genes with a common response across the 5 genotypes (83 genes) were associated with photosynthesis, in particular photosystem I. The functional annotation of genes not differentially expressed in B73 but responsive in the other 4 genotypes (789) identifies reactive oxygen species. This suggests that B73 has a different response to long-term ozone exposure than the other 4 genotypes. The relative magnitude of the genotypic response to ozone, and the enrichment analyses are consistent regardless of whether aligning short reads to: long read assembled transcripts; the B73 reference; the Mo17 reference. We find that prolonged ozone exposure directly impacts the photosynthetic machinery of the leaf.