Sulfonamide-Bridged Nucleic Acid: Synthesis, High RNA Selective Hybridization, and High Nuclease Resistance
Sulfonamide-Bridged Nucleic Acid: Synthesis, High RNA Selective Hybridization, and High Nuclease Resistance
复制标题
磺酰胺桥核酸：合成、高 RNA 选择性杂交和高核酸酶抗性
DOI：
10.1021/ol503029v
复制
发表时间：
2014
期刊：
影响因子：
5.2
通讯作者：
S. Obika
中科院分区：
文献类型：
--
作者：
Y. Mitsuoka;Y. Fujimura;R. Waki;A. Kugimiya;T. Yamamoto;Y. Hari;S. Obika
合成了2 ' - n,4 ' - c -（n -甲氨基）磺酰基亚甲基-桥接胸苷（SuNA），它具有一个六元的连键，并引入到寡核苷酸中。与天然寡核苷酸相比，含有SuNA的寡核苷酸表现出优异的核酸酶抗性，对单链RNA具有高亲和力，对单链DNA具有低亲和力。
2′-N,4′-C-(N-Methylamino)sulfonylmethylene-bridged thymidine (SuNA), which has a six-membered linkage including a sulfonamide moiety, was synthesized and introduced into oligonucleotides. The oligonucleotides containing SuNA exhibited excellent nuclease resistance, a high affinity toward single-stranded RNA, and a low affinity toward single-stranded DNA compared to the natural oligonucleotide.