Specific binding sites for the phagocytosis stimulating peptide tuftsin on human polymorphonuclear leukocytes and monocytes.
Specific binding sites for the phagocytosis stimulating peptide tuftsin on human polymorphonuclear leukocytes and monocytes.
复制标题
吞噬作用刺激肽 tuftsin 在人多形核白细胞和单核细胞上的特异性结合位点。
DOI：
10.1016/0006-291x(78)91032-x
复制
发表时间：
1978
影响因子：
3.1
通讯作者：
M. Fridkin
中科院分区：
文献类型：
--
作者：
Y. Stabinsky;P. Gottlieb;V. Zakuth;Z. Spirer;M. Fridkin
合成了比活度为9Ci/mmo1的高纯度和生物活性的[~3H]Tuftsin，并在22℃下研究了其与几种类型的人循环血细胞的结合。由平衡实验得到的离解常数(Kd)分别为130和125 nm。每个细胞的结合位点数分别约为50,000和100,000。在相同的实验条件下，淋巴细胞对[~3H]Tuftsin的结合能力只有一个阈值，而红细胞则没有检测到结合。
Highly purified and biologically active [3H]tuftsin (specific activity 9 Ci/mmol) was synthesized and its binding to several types of human circulating blood cells was studied at 22°C. The binding to polymorphonuclear leukocytes and to monocytes was found to be specific, fast, saturable and reversible. Values for the dissociation constants (KD) were derived from equilibrium experiments and are 130 and 125 nM, respectively. The number of binding sites is approximately 50,000 and 100,000 per cell, respectively. Under the same experimental conditions lymphocytes exhibited only a threshold binding capacity for [3H]tuftsin whereas erythrocytes revealed no detectable binding.