MiR-324-5p assists ultrasonography in predicting lymph node metastasis of unifocal papillary thyroid microcarcinoma without extracapsular spread.
MiR-324-5p assists ultrasonography in predicting lymph node metastasis of unifocal papillary thyroid microcarcinoma without extracapsular spread.
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MiR-324-5p辅助超声预测无包膜外扩散的单灶性甲状腺微小乳头状癌的淋巴结转移
DOI：
10.18632/oncotarget.19717
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发表时间：
2017-10-13
期刊：
影响因子：
--
通讯作者：
Zhan W
中科院分区：
文献类型：
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作者：
Yang Y;Xia S;Ni X;Ni Z;Zhang L;Wang W;Kong Y;Wang Y;Ye L;Zhan W
超声检查是甲状腺乳头状癌淋巴结转移的首选检查方法，对甲状腺乳头状癌的治疗选择至关重要。但超声检查对PTC淋巴结转移的敏感性较低，尤其是中央淋巴结转移。据报道，miR-324- 5 p在各种癌症的转移中起重要作用。为探讨PTC中miR-324- 5 p与LNM的关系，采用实时荧光定量聚合酶链反应（real-time polymerase chain reaction，RT-PCR）技术对PTC组织进行检测。并与PTC的LNM进行相关性分析。并对与淋巴结转移有关的临床病理因素和超声因素进行了分析。此外，还进行了PTC细胞中miR-324- 5 p的功能测定。目前研究表明，年龄是LNM的独立保护因素，多灶性、TNM分期晚、甲状腺结节横径增大、超声怀疑LNM是LNM的独立危险因素。miR-324- 5 p促进PTC细胞增殖、迁移和侵袭。miR-324- 5 p可作为预测LNM的候选预测因子，沿着超声检查可预测无包膜外扩散的单灶性甲状腺乳头状微小癌的LNM，尤其是中央LNM。
Ultrasonography is the first choice of lymph node metastasis (LNM) detection which is crucial for therapeutic options of papillary thyroid cancer (PTC). However, the sensitivity of ultrasonography in detecting LNM of PTC is relatively low; especially in central LNM. MiR-324-5p has been reported to play important roles in the metastasis of various cancers. To explore the relationship between miR-324-5p and LNM in PTC, quantitative real-time polymerase chain reaction was performed in PTC tissue and fine needle aspiration (FNA) washout successively. Its correlation with LNM of PTC was analyzed. The clinicopathological and sonographic factors relating to LNM were also studied. Additionally, the function assay of miR-324-5p in PTC cells was conducted. Current study demonstrated that age was an independent protective factor and multifocality, advanced TNM stage, increased transverse diameter of thyroid nodule, ultrasound suspected LNM were independent risk factors of LNM. MiR-324-5p promoted proliferation, migration and invasion of PTC cell line. MiR-324-5p could serve as a candidate predictor along with ultrasonography in predicting LNM, especially central LNM of unifocal papillary thyroid microcarcinoma without extracapsular spread.