Functionalization of titanium implants using a modular system for binding and release of VEGF enhances bone-implant contact in a rodent model.
Functionalization of titanium implants using a modular system for binding and release of VEGF enhances bone-implant contact in a rodent model.
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DOI：
10.1111/jcpe.12370
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发表时间：
2015-03
影响因子：
6.7
通讯作者：
H. Schliephake;J. Rublack;A. Förster;B. Schwenzer;J. Reichert;D. Scharnweber
中科院分区：
文献类型：
--
作者：
H. Schliephake;J. Rublack;A. Förster;B. Schwenzer;J. Reichert;D. Scharnweber
目的探讨血管内皮生长因子165（VEGF 165）在钛种植体表面的DNA寡核苷酸（DNA oligonucleotide，ODN）锚链固定化对种植体周围骨形成的促进作用。材料与方法将DNA寡核苷酸锚定在喷砂酸蚀（SAE）钛螺钉植入物的表面，并与结合rhVEGF 165的ODN互补链杂交。对照空白SAE植入物和具有纳米锚定ODN的SAE植入物测试植入物。将植入物插入36只Sprague-Dawley大鼠的胫骨中。主要结局参数为骨-种植体接触（BIC）、新骨形成量和种植体周围骨密度（BD）。密度后1，4和13周。分析单位为单个植入物。结果与空白种植体和锚定ODN链种植体相比，与ODN锚链杂交的rhVEGF 165种植体在1个月后显示出显著增加的平均BIC。结论纳米锚定寡核苷酸链将rhVEGF 165固定于钛种植体表面，可在一定程度上促进喷砂和酸蚀钛种植体的BIC。生长因子的作用半径似乎仅限于紧邻植入物表面的组织。
AIMS To test the immobilization of vascular endothelial growth factor (VEGF165 ) on the surface of titanium implants using DNA oligonucleotide (ODN) anchor strands for the ability to enhance periimplant bone formation. MATERIALS AND METHODS DNA oligonucleotides were anchored to the surface of sandblasted acid-etched (SAE) titanium screw implants and were hybridized with complementary strands of ODN conjugated to rhVEGF165 . The implants were tested against blank SAE implants and SAE implants with nano-anchored ODN. The implants were inserted into the tibiae of 36 Sprague-Dawley rats. Primary outcome parameters were bone-implant contact (BIC), amount of new bone formation and periimplant bone density (BD). density after 1, 4 and 13 weeks. Unit of analysis has been the individual implant. RESULTS Implants with rhVEGF165 hybridized to ODN anchor strands exhibited significantly increased average BIC after 1 month compared to blank implants and implants with anchored ODN strands. CONCLUSIONS It is concluded that rhVEGF165 immobilized on the surface of titanium implants through nano-anchored oligonucleotide strands can accelerate BIC of sandblasted and etched titanium implants to a certain extent. The radius of effect of the growth factor appears to be limited to tissue immediately adjacent to the implant surface.