Applications of one-Step environmentally-friendly fermentation method to produce fumaric acid with immobilized Rhizopus arrhizus in stirred-tank reactor
Applications of one-Step environmentally-friendly fermentation method to produce fumaric acid with immobilized Rhizopus arrhizus in stirred-tank reactor
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DOI：
10.15376/biores.16.4.8197-8204
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发表时间：
2021-10
期刊：
影响因子：
1.5
通讯作者：
Yuchen Ning;Yang Li;Shijie Zhao;Huan Liu;Li Deng;Fang Wang
中科院分区：
文献类型：
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作者：
Yuchen Ning;Yang Li;Shijie Zhao;Huan Liu;Li Deng;Fang Wang
传统的真菌两步培养模式通常分为种子阶段（通常为24 ~ 48 h）和生产阶段（通常为96 ~ 144 h）。两个阶段的使用延长了整个生产周期，并产生了多余的废水。本研究采用一种高效环保的一步发酵方法，在搅拌槽反应器中固定化生根根霉生产富马酸。优化了一步发酵过程中氮源含量和反应器搅拌速度这两个关键因素；富马酸产量为51 g/L，葡萄糖产率为0.42 g/g，与传统两步发酵工艺的产物相当。此外，总生产周期缩短至96 h，并且由于避免了种子培养过程而减少了废水量。因此，利用一步发酵法生产富马酸具有较好的可行性和环保性。这是利用真菌工业化生产富马酸和其他高价值生化物质的一种很有前途的方法。
The traditional two-step cultivation mode for fungal cultivation is commonly divided into the seed stage (usually 24 to 48 h) and the production stage (usually 96 to 144 h). The use of two stages prolongs the total production cycle and generates excess wastewater. In this work, an efficient and environmentally friendly one-step fermentation method was applied for the production of fumaric acid by immobilized Rhizopus arrhizus in a stirred-tank reactor. The nitrogen source content and the agitation speed of the reactor were optimized as the two critical factors in the one-step fermentation process; the fumaric acid production of 51 g/L with a yield of 0.42 g/g glucose was comparable with the product from the traditional two-step fermentation process. Furthermore, the total production cycle was shortened to 96 h, and the amount of wastewater was reduced due to the avoidance of the seed culture process. Thus, utilization of the one-step fermentation method to produce fumaric acid was shown to be preferable feasibility and environmental-friendliness. This is a promising method for industrial production of fumaric acid and other high-value biochemicals by fungus.