The Arabidopsis gene hypersensitive to phosphate starvation 3 encodes ethylene overproduction 1.
The Arabidopsis gene hypersensitive to phosphate starvation 3 encodes ethylene overproduction 1.
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DOI：
10.1093/pcp/pcs072
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发表时间：
2012-06
影响因子：
4.9
通讯作者：
Liangsheng Wang;Jinsong Dong;Ziyue Gao;Dong Liu
中科院分区：
文献类型：
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作者：
Liangsheng Wang;Jinsong Dong;Ziyue Gao;Dong Liu
当植物受到无机磷酸盐（Pi）缺乏时，它们表现出一系列反应来应对这种营养胁迫。在这项工作中，我们鉴定了两个拟南芥突变体hps3-1和hps3-2（对Pi饥饿3超敏感），它们在Pi充足或缺乏条件下生长时改变了Pi饥饿诱导（PSI）基因的表达并增强了酸性磷酸酶（APase）的产生。然而，hps3-1和hps3-2在缺乏pi的培养基上生长时，积累的花青素比野生型少。分子克隆表明，hps3突变体的表型是由ETO1（乙烯过量生产1）基因突变引起的。在拟南芥中，ETO1编码乙烯生物合成的负调控因子，ETO1的突变导致拟南芥幼苗过量产生乙烯。乙烯生物合成抑制剂氨基乙氧基乙烯基甘氨酸或乙烯感知抑制剂Ag（+）抑制了hps3的所有突变型。综上所述，这些结果提供了进一步的遗传证据，证明乙烯是多种植物对Pi饥饿反应的重要调节因子。此外，我们发现乙烯水平的变化对PSI基因的表达、Pi稳态的维持、APase的产生和花青素的积累有不同的影响。我们还证明了乙烯信号主要调节根表面相关APase的活性，而不是总APase活性。
When plants are subjected to a deficiency in inorganic phosphate (Pi), they exhibit an array of responses to cope with this nutritional stress. In this work, we have characterized two Arabidopsis mutants, hps3-1 and hps3-2 (hypersensitive to Pi starvation 3), that have altered expression of Pi starvation-induced (PSI) genes and enhanced production of acid phosphatase (APase) when grown under either Pi sufficiency or deficiency conditions. hps3-1 and hps3-2, however, accumulate less anthocyanin than the wild type when grown on a Pi-deficient medium. Molecular cloning indicated that the phenotypes of hps3 mutants were caused by mutations within the ETO1 (ETHYLENE OVERPRODUCTION 1) gene. In Arabidopsis, ETO1 encodes a negative regulator of ethylene biosynthesis, and mutation of ETO1 causes Arabidopsis seedlings to overproduce ethylene. The ethylene biosynthesis inhibitor aminoethoxyvinyl glycine or the ethylene perception inhibitor Ag(+) suppressed all the mutant phenotypes of hps3. Taken together, these results provide further genetic evidence that ethylene is an important regulator of multiple plant responses to Pi starvation. Furthermore, we found that a change in ethylene level has differential effects on the expression of PSI genes, maintenance of Pi homeostasis, production of APase and accumulation of anthocyanin. We also demonstrated that ethylene signaling mainly regulates the activity of root surface-associated APases rather than total APase activity.