Measuring Feedforward Inhibition and Its Impact on Local Circuit Function.
Measuring Feedforward Inhibition and Its Impact on Local Circuit Function.
复制标题
测量前馈抑制及其对局部电路功能的影响。
DOI：
10.1101/pdb.prot095828
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发表时间：
2017
影响因子：
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通讯作者：
Hull,Court
中科院分区：
文献类型：
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作者：
Hull,Court
该协议描述了一系列的方法来测量前馈抑制在急性脑切片从小脑皮质。使用浦肯野细胞的全细胞电压和电流钳记录，沿着平行纤维的电刺激，这些方法将演示如何测量前馈电路中兴奋和抑制之间的关系。此外，该方案将描述如何测量前馈抑制对浦肯野细胞兴奋性的影响，重点是尖峰时间。
This protocol describes a series of approaches to measure feedforward inhibition in acute brain slices from the cerebellar cortex. Using whole cell voltage and current clamp recordings from Purkinje cells, along with electrical stimulation of the parallel fibers, these approaches will demonstrate how to measure the relationship between excitation and inhibition in a feedforward circuit. In addition, this protocol will describe how to measure the impact of feedforward inhibition on Purkinje cell excitability, with an emphasis on spike timing.
准备脑切片以研究基本突触特性。
影响因子：
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作者：
M. A. Xu
通讯作者：
M. A. Xu