Preparation, Separation and Antioxidant Properties of Hydrolysates Derived from Grifola frondosa Protein
Preparation, Separation and Antioxidant Properties of Hydrolysates Derived from Grifola frondosa Protein
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灰树花蛋白水解物的制备、分离及抗氧化性能
DOI：
10.17221/197/2015-cjfs
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发表时间：
2015-01-01
影响因子：
1.3
通讯作者：
Chen, Guitang
中科院分区：
文献类型：
--
作者：
Dong, Yaru;Qi, Guohong;Chen, Guitang
为了充分利用灰树花资源，从灰树花子实体中提取蛋白质（GFP），并使用六种不同的蛋白酶（木瓜蛋白酶、中性酶、碱性酶、胰蛋白酶、胃蛋白酶和Protamex）消化产生抗氧化水解产物。 1.5 h制备的胰蛋白酶水解液在不同水解液中具有最强的抗氧化能力，通过不同截留分子量（MWCO）的超滤膜将其分离成四个主要部分，即GFHT-1（Mw > 10 kDa）、GFHT-2（Mw = 5-10 kDa）、GFHT-3（Mw = 3-5 kDa）和GFHT-4（Mw < 3 kDa）。随后进一步评价了4个馏分的体外抗氧化活性，包括对DPPH的清除作用、亚铁离子螯合作用、还原力以及抑制亚油酸自氧化的能力。结果表明，所有组分都是有效的抗氧化剂，其中 GFHT-4 具有最高的抗氧化活性。然后通过凝胶过滤色谱将GFHT-4分离成两个主要肽级分，这两个级分分别位于2385Da(F-1)和1138Da(F-2)。
In order to take full advantage of Grifola frondosa resources, the protein (GFP) was extracted from G. frondosa fruiting body and digested using six different proteases (papain, neutrase, alcalase, trypsin, pepsin, and Protamex) to produce the antioxidative hydrolysates. The trypsin hydrolysate prepared at 1.5 h possessed the strongest antioxidant potential among different hydrolysates, which was separated into four major fractions by ultrafiltration membranes with different molecular weight cut-off (MWCO), that are GFHT-1 (Mw > 10 kDa), GFHT-2 (Mw = 5-10 kDa), GFHT-3 (Mw = 3-5 kDa), and GFHT-4 (Mw < 3 kDa). In succession, the in vitro antioxidant activities of the four fractions were further evaluated, including the scavenging effect on DPPH, ferrous ion chelating effect, reducing power, and ability to inhibit the autoxidation of linoleic acid. The results demonstrated that all fractions are effective antioxidants and comparably GFHT-4 has the highest antioxidant activity. Then GFHT-4 was separated to two major peptide fractions by gel filtration chromatography, and the two fractions were located at 2385 Da (F-1) and 1138 Da (F-2), respectively.