A microfabricated array bioreactor for perfused 3D liver culture
A microfabricated array bioreactor for perfused 3D liver culture
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DOI：
10.1002/bit.10143
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发表时间：
2002-05-05
影响因子：
3.8
通讯作者：
Griffith, LG
中科院分区：
文献类型：
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作者：
Powers, MJ;Domansky, K;Griffith, LG
我们描述了生物反应器的设计、制造和性能，该生物反应器能够在连续灌注下实现 3D 组织结构的形态发生，并通过光学显微镜进行重复原位观察。三维支架是通过对硅片进行深度反应离子蚀刻来创建的，以创建具有细胞粘附壁的通道（通孔）阵列。支架与反应器外壳中的细胞保留过滤器和支架相结合，旨在将连续的灌注液输送到阵列顶部并穿过每个通道中的 3D 组织块。反应器尺寸的构建使得灌注液流速满足细胞需氧量的估计值，同时提供等于或低于生理范围的流体剪切应力（
We describe the design, fabrication, and performance of a bioreactor that enables both morphogenesis of 3D tissue structures under continuous perfusion and repeated in situ observation by light microscopy. Three-dimensional scaffolds were created by deep reactive ion etching of silicon wafers to create an array of channels (through-holes) with cell-adhesive walls. Scaffolds were combined with a cell-retaining filter and support in a reactor housing designed to deliver a continuous perfusate across the top of the array and through the 3D tissue mass in each channel. Reactor dimensions were constructed so that perfusate flow rates meet estimated values of cellular oxygen demands while providing fluid shear stress at or below a physiological range (