Using liquid chromatography-tandem mass spectrometry to quantify mono hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine
Using liquid chromatography-tandem mass spectrometry to quantify mono hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine
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DOI：
10.1016/j.jchromb.2009.02.067
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发表时间：
2009-04-15
影响因子：
3
通讯作者：
Waidyanatha, Suramya
中科院分区：
文献类型：
--
作者：
Onyemauwa, Frank;Rappaport, Stephen M.;Waidyanatha, Suramya
我们提出了一种测定方法，采用酶消化和固相萃取，然后通过液相色谱-串联质谱同时定量16羟基化多环芳烃（OHPAH）在3毫升的尿液样品。分析物由2-，3-和4-环OHPAH，即1-和2-羟基萘（1-和2-OHNAP），2-羟基氟（2-OHFLU）组成。1-、2-、3-、4-和9-羟基菲（1-、2-、3-、4-和9-OHPHE）、1-羟基芘（1-OHPYR）、1-和2-羟基苯并（a）蒽（1-和2-OHBAA）、3-和6-羟基蒽（3-和6-OHCHR）以及3-、7-和9-羟基苯并（a）芘（3-、7-和9-OHBAP）。用钢铁工人和对照组的尿样对该方法进行了验证。特定分析物的方法变异系数在7%至27%之间，定量限在0.002至0.010 μ g/L尿液之间。2-环和3-环OHPAH在所有受试者中很容易定量。然而，1-OHPYR是可定量的4-和5-环代谢物的唯一代表。两两相关分析表明，所有的OHPAHs都高度相关（0.553
We present an assay which employs enzyme digestion and solid phase extraction followed by liquid chromatography-tandem mass spectrometry to simultaneously quantify 16 hydroxylated polycyclic aromatic hydrocarbons (OHPAHs) in 3-ml samples of urine. The analytes consisted of 2-, 3-, and 4-ring OHPAHs, namely, 1- and 2-hydroxynaphthalene (1- and 2-OHNAP), 2-hydroxyfluorine (2-OHFLU). 1-, 2-, 3-, 4-, and 9-hydroxyphenanthrene (1-, 2-, 3-, 4-, and 9-OHPHE), 1-hydroxypyrene (1-OHPYR), 1- and 2-hydroxybenzo(a)anthracene (1- and 2-OHBAA), 3- and 6-hydroxychrysene (3- and 6-OHCHR) and 3-, 7-, and 9-hdroxbenzo(a)pyrene (3-, 7-, and 9-OHBAP). The method was validated using urine samples from steel workers and control subjects. The coefficients of variation of the method for the particular analytes were between 7% and 27% and the limits of quantitation were between 0.002 and 0.010 mu g/l urine. The 2- and 3-ring OHPAHs were easily quantified in all subjects. However, 1-OHPYR was the only representative of the 4- and 5-ring metabolites that could be quantified. Pairwise correlations showed that all OHPAHs were highly correlated with each other (0.553