Metatranscriptomic analysis reveals active microbes and genes responded to short-term Cr(VI) stress.
Metatranscriptomic analysis reveals active microbes and genes responded to short-term Cr(VI) stress.
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宏转录组分析揭示了活性微生物和基因对短期 Cr(VI) 应激的反应。
DOI：
10.1007/s10646-020-02290-5
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发表时间：
2020
期刊：
影响因子：
2.7
通讯作者：
Xiangkai Li
中科院分区：
文献类型：
--
作者：
Zhengsheng Yu;Yaxin Pei;Shuai Zhao;Apurva Kakade;Aman Khan;Monika Sharma;Hajira Zain;Pengya Feng;Jing Ji;Tuoyu Zhou;Haoyang Wang;Jingyuan Wu;Xiangkai Li
重金属对环境造成了严重的污染。然而，微生物群落对短期重金属胁迫的响应机制尚不清楚。本研究采用宏基因组学和元转录组学的方法，观察了微生物对短期Cr（VI）胁迫的反应。MG数据显示，对照组和Cr（VI）处理组中99.1%的物种相似。然而，MT数据表明，在短期Cr（VI）孵育后，83%的微生物具有活性，其中58.7%增加，而41.3%的相对丰度下降。MT结果还显示，样品中有9%的微生物处于休眠状态。与氧化应激、Cr（VI）转运、抗性和还原相关的基因以及功能未知的基因在Cr（VI）处理后上调2-10倍。为了进一步证实未知基因的功能，选择了两个基因（314和494）检测其对Cr（VI）的抗性和还原能力。结果表明，这些基因显著提高了大肠杆菌对Cr（VI）的修复能力. MT结果还显示，在Cr（VI）处理后，一些稀有属的表达增加（至少两倍），表明这些稀有物种在微生物对短期Cr（VI）胁迫的反应中起着至关重要的作用。总之，MT是了解特定环境条件下活性和休眠微生物作用的有效方法。
Heavy metals have been severely polluting the environment. However, the response mechanism of microbial communities to short-term heavy metals stress remains unclear. In this study, metagenomics (MG) and metatranscriptomics (MT) was performed to observe the microbial response to short-term Cr(VI) stress. MG data showed that 99.1% of species were similar in the control and Cr(VI) treated groups. However, MT data demonstrated that 83% of the microbes were active in which 58.7% increased, while the relative abundance of 41.3% decreased after short-term Cr(VI) incubation. The MT results also revealed 9% of microbes were dormant in samples. Genes associated with oxidative stress, Cr(VI) transport, resistance, and reduction, as well as genes with unknown functions were 2–10 times upregulated after Cr(VI) treatment. To further confirm the function of unknown genes, two genes (314and494) were selected to detect the Cr(VI) resistance and reduction ability. The results showed that these genes significantly increased the Cr(VI) remediation ability ofEscherichia coli. MT results also revealed an increase in the expression of some rare genera (at least two times) after Cr(VI) treatment, indicating these rare species played a crucial role in microbial response to short-term Cr(VI) stress. In summary, MT is an efficient way to understand the role of active and dormant microbes in specific environmental conditions.