Antibodies to sulfatide in cerebrospinal fluid of patients with multiple sclerosis
Antibodies to sulfatide in cerebrospinal fluid of patients with multiple sclerosis
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DOI：
10.1016/s0165-5728(03)00131-0
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发表时间：
2003-06-01
影响因子：
3.3
通讯作者：
Cook, SD
中科院分区：
文献类型：
--
作者：
Ilyas, AA;Chen, ZW;Cook, SD
在多发性硬化症（MS）的目标抗原(s)的身份仍然难以捉摸，尽管许多努力确定它。采用酶联免疫吸附试验（ELISA）和薄层色谱（TLC）免疫染色技术，对MS、其他神经系统疾病（OND）、其他疾病（OD）和健康对照者的脑脊液（CSF）进行了抗纯化硫脂（人髓磷脂的主要糖鞘脂）抗体的分析。MS患者的抗硫脂抗体水平明显高于OND组（p < 0.05）和所有对照组（p < 0.025）。用tlc免疫染色证实了高滴度抗体与硫脂的结合。在NIS的所有亚型中均检测到抗硫脂抗体，尽管继发性进展型MS （SPMS）患者的频率高于原发性进展型MS （PPMS）和复发缓解型MS （RRMS）患者。数据显示多发性硬化症患者脑脊液中对硫脂的体液反应(C) 2003 Elsevier Science B.V.。
The identity of target antigen(s) in multiple sclerosis (MS) remains elusive despite much effort to identify it. We analyzed cerebrospinal fluid (CSF) from patients with MS, other neurological diseases (OND), other diseases (OD) and healthy controls for antibodies against purified sulfatide, a major glycosphingolipid of human myelin, by an enzyme-linked immunosorbent assay (ELISA) and a thin-layer chromatogram (TLC)-immunostaining technique. Elevated anti-sulfatide antibodies were significantly higher in MS patients as compared with the OND group (p < 0.05) and all controls combined (P < 0.025). Binding of high titer antibodies to sulfatide was confirmed with TLC-immunostaining. Anti-sulfatide antibodies were detected in all subtypes of NIS although the frequency was higher in patients with secondary progressive MS (SPMS) than in patients with primary progressive (PPMS) and relapsing-remitting MS (RRMS). The data demonstrate a humoral response to sulfatide in the CSF of patients with MS. (C) 2003 Elsevier Science B.V. All rights reserved.