The expression and localization of a novel protein phosphatase inhibitor 2810408A11Rik in mouse testis and sperm
The expression and localization of a novel protein phosphatase inhibitor 2810408A11Rik in mouse testis and sperm
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DOI：
10.1016/s1674-8301(12)60020-7
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发表时间：
2012-02-01
影响因子：
2.3
通讯作者：
Sha, Jiahao
中科院分区：
文献类型：
--
作者：
Bi, Ye;Liu, Mingxi;Sha, Jiahao
本研究观察了2810408 A11 Rik在小鼠睾丸和精子中的表达和分布，探讨其在精子发生和精子功能中的作用。利用生物信息资源和RT-PCR技术分析了多个组织样本中2810408 A11 Rik mRNA的表达水平。通过原核表达2810408 A11 Rik重组蛋白，制备了特异性兔多克隆抗体，并用于动物免疫。采用Western blotting、免疫组化和免疫荧光法检测2810408 A11 Rik在正常肝组织中的表达和分布。生物信息学分析和RT-PCR结果表明，2810408 A11 Rik mRNA在小鼠睾丸中特异性表达，2810408 A11 Rik蛋白含有蛋白磷酸酶抑制剂结构域。Western blotting、免疫组化和免疫荧光检测证实2810408 A11 Rik蛋白在小鼠睾丸中表达，尤其在减数分裂后的圆形和长形精子细胞中，且定位于精子的顶体和核后区。我们的研究结果表明，2810408 A11 Rik可能在精子发生，精子获能和受精中发挥重要作用。
This study investigated the expression and distribution of 2810408A11Rik in mouse testis and sperm, and explored its role in spermatogenesis and sperm function. The expression levels of 2810408A11Rik mRNA in multiple tissue samples were analyzed using bioinformatic resources and RT-PCR technique. A specific rabbit polyclonal antibody was prepared by prokaryotic expression of 2810408A11Rik recombinant protein and utilized for animal immunization. Western blotting, immunohistochemistry and immunofluorescence were used to detect the expression and distribution of 2810408A11Rik. The results of the bioinformatic analysis and RT-PCR showed that 2810408A11Rik mRNA was specifically expressed in mouse testis, and 2810408A11Rik protein included a protein phosphatase inhibitor domain. Western blotting assays, immunohistochemistry and immunofluorescence confirmed the expression of 2810408A11Rik protein in mouse testis, especially in post-meiosis round and long spermatids, and that it is localized in the acrosome and the post-nucleus area of sperm. Our findings suggest that 2810408A11Rik may play an important role in spermatogenesis, sperm capacitation and fertilization.