A synthetic biochemistry platform for cell free production of monoterpenes from glucose.
A synthetic biochemistry platform for cell free production of monoterpenes from glucose.
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DOI：
10.1038/ncomms15526
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发表时间：
2017-05-24
影响因子：
16.6
通讯作者：
Bowie JU
中科院分区：
文献类型：
--
作者：
Korman TP;Opgenorth PH;Bowie JU
旨在将生物质复杂地化学转化为生物燃料或商品化学品的无细胞系统正在成为活细胞代谢工程的有前途的替代方案。在这里，我们设计了一个系统，包括27种酶，用于将葡萄糖转化为单萜，在修饰的葡萄糖分解模块中产生NAD（P）H和ATP，并利用这两种辅因子来构建萜烯。在我们的系统中，通过改变萜烯合酶来产生不同的单萜烯。该系统对于柠檬烯、蒎烯和桧烯的生产是稳定的，并且可以从单次添加葡萄糖开始连续操作至少5天。我们得到的转化率>95%，滴度>15 g l−1。滴度是超过细胞毒性限度的一个数量级，因此难以使用基于细胞的系统实现。总的来说，这些结果突出了合成生物化学方法生产生物基化学品的潜力。萜类化合物是一种具有生物活性的天然产物，具有多种商业用途。在这里，作者设计了一个由27种酶组成的无细胞系统，将葡萄糖转化为萜烯，突出了合成生物化学方法用于生物化合物生产的潜力。
Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells. Here we design a system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes. Different monoterpenes are produced in our system by changing the terpene synthase enzyme. The system is stable for the production of limonene, pinene and sabinene, and can operate continuously for at least 5 days from a single addition of glucose. We obtain conversion yields >95% and titres >15 g l−1. The titres are an order of magnitude over cellular toxicity limits and thus difficult to achieve using cell-based systems. Overall, these results highlight the potential of synthetic biochemistry approaches for producing bio-based chemicals. Terpenes are bioactive natural products derived from plants with several commercial applications. Here, the authors engineer a cell-free system composed of 27 enzymes that convert glucose into terpenes, highlighting the potential of synthetic biochemistry approaches for biocompounds production.