A new face on apoptosis: death-associated protein 3 and PDCD9 are mitochondrial ribosomal proteins
A new face on apoptosis: death-associated protein 3 and PDCD9 are mitochondrial ribosomal proteins
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DOI：
10.1016/s0014-5793(01)02250-5
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发表时间：
2001-03-09
期刊：
影响因子：
3.5
通讯作者：
Spremulli, LL
中科院分区：
文献类型：
--
作者：
Koc, EC;Ranasinghe, A;Spremulli, LL
已知参与促进哺乳动物细胞凋亡的酪氨酸蛋白已被鉴定为哺乳动物线粒体核糖体的组分。对整个线粒体核糖体亚基进行蛋白水解消化，然后使用液相色谱-串联质谱法分析存在的肽，揭示了促凋亡蛋白、死亡相关蛋白3（DAP 3）和程序性细胞死亡蛋白9，都是线粒体核糖体的组成部分DAP 3具有鸟嘌呤核苷酸结合蛋白的特征基序，并且可能是解释哺乳动物线粒体核糖体的核苷酸结合活性的蛋白质。本文报道的观察结果暗示线粒体蛋白合成是细胞凋亡信号通路的主要组分。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
Tyro proteins known to be involved in promoting apoptosis in mammalian cells have been identified as components of the mammalian mitochondrial ribosome, Proteolytic digestion of whole mitochondrial ribosomal subunits followed by analysis of the peptides present using liquid chromatography-tandem mass spectrometry revealed that the proapoptotic proteins, death-associated protein 3 (DAP3) and the programmed cell death protein 9, are both components of the mitochondrial ribosome. DAP3 has motifs characteristic of guanine nucleotide binding proteins and is probably the protein that accounts for the nucleotide binding activity of mammalian mitochondrial ribosomes, The observations reported here implicate mitochondrial protein synthesis as a major component in cellular apoptotic signaling pathways. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.