Generation of Rat Monoclonal Antibodies Against Human Epidermal Growth Factor Receptor 2
Generation of Rat Monoclonal Antibodies Against Human Epidermal Growth Factor Receptor 2
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抗人表皮生长因子受体2的大鼠单克隆抗体的产生
DOI：
10.1089/mab.2022.0042
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发表时间：
2023
影响因子：
--
通讯作者：
Yokoyama Chikako
中科院分区：
文献类型：
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作者：
Yamamoto Honoka;Nakanishi Takeshi;Ihara Kan-ichiro;Tachibana Taro;Yokoyama Chikako
人表皮生长因子受体2(HER2)是一种没有任何已知配体的跨膜酪氨酸激酶受体，属于表皮生长因子受体(EGFR)家族。它是一种原癌蛋白，通过与其他EGFR家族受体的同源和异源二聚化，通过信号级联反应促进细胞增殖，抑制癌细胞凋亡。由于包括乳腺癌在内的几种癌症都过表达HER2，因此它是肿瘤治疗的靶点。曲妥珠单抗和pertuzumab都是重组人源化单抗(MAbs)，用于针对HER2胞外区(ECD)的临床试验。因此，产生针对HER2的各种ECDs的抗体是很重要的。在这项研究中，我们描述了针对人HER2的ECD而产生的鼠单抗。人乳腺癌细胞系SK-BR-3由于表达HER2而进行免疫荧光染色，mAbs可以在细胞内检测到完整的和内源性的HER2。
Human epidermal growth factor receptor 2 (HER2) is a transmembrane tyrosine kinase receptor without any known ligands and a member of the epidermal growth factor receptor (EGFR) family. It is a proto-oncogenic protein that, through signaling cascades, promotes cell proliferation and inhibits apoptosis in cancer cells through homo- and heterodimerization with other EGFR family receptors. Since several cancers, including breast cancer, overexpress HER2, it is a target of tumor therapy. Both trastuzumab and pertuzumab are recombinant humanized monoclonal antibodies (mAbs) used in clinical trials that target the extracellular domain (ECD) of HER2. Therefore, it is important to generate antibodies against various ECDs of HER2. In this study, we describe rat mAbs, which were generated against the ECD of human HER2. The human breast cancer cell line SK-BR-3 was subjected to immunofluorescence staining as it expresses HER2, and mAbs can detect both intact and endogenous HER2 within the cell line.