Lymphoid apoptosis in Edwardsiella tarda septicemia in tilapia, Oreochromis niloticus.
Lymphoid apoptosis in Edwardsiella tarda septicemia in tilapia, Oreochromis niloticus.
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DOI：
10.1016/j.fsi.2006.08.007
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发表时间：
2007-06
影响因子：
4.7
通讯作者：
N. Pirarat;M. Maita;M. Endo;T. Katagiri
中科院分区：
文献类型：
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作者：
N. Pirarat;M. Maita;M. Endo;T. Katagiri
本研究揭示了细胞凋亡的动力学变化与实验性腹腔感染迟缓爱德华氏菌作为败血症模型的炎症反应之间的关系。光镜和透射电镜观察凋亡细胞的形态学变化，包括细胞皱缩、核染色质浓缩、核碎裂和膜泡形成。TUNEL和琼脂糖凝胶电泳证实了凋亡细胞中DNA的断裂。在炎症过程之前，在淋巴器官中高度检测到细胞凋亡，并在广泛的炎症反应后逐渐减少。胸腺和脾脏的细胞凋亡是广泛的，体外研究表明，淋巴细胞是发生细胞凋亡的主要细胞群。结果表明，E.迟发性通过淋巴细胞凋亡诱导的全身性免疫抑制，如通过全身性败血症初始步骤期间全身性炎症反应的抑制所确定的。
The present study revealed a relationship between the kinetic change of apoptosis and the inflammatory response during experimental intraperitoneal infection with Edwardsiella tarda as a septicemic model. The morphological changes of apoptotic cells including cellular shrinkage, condensed nuclear chromatin, nuclear fragmentation and membrane blebbing were detected by light and transmission electron microscopy. TUNEL and agarose gel electrophoresis confirmed the fragmentation of DNA in the apoptotic cells. Apoptosis was highly detected in lymphoid organs prior to the inflammatory process and gradually decreased after an extensive inflammatory response. Apoptosis in thymus and spleen was extensive and an in vitro study revealed that lymphocytes were the major cell population which underwent apoptosis. The result suggests that E. tarda-induced systemic immunosuppression via lymphocyte apoptosis as determined by suppression of the systemic inflammatory response during an initial step of generalized septicemia.