IMMUNOREACTIVE DYNORPHIN IN MAMMALIAN SPINAL-CORD AND DORSAL ROOT-GANGLIA
IMMUNOREACTIVE DYNORPHIN IN MAMMALIAN SPINAL-CORD AND DORSAL ROOT-GANGLIA
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DOI：
10.1073/pnas.78.12.7783
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
GOLDSTEIN, A
中科院分区：
文献类型：
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作者：
BOTTICELLI, LJ;COX, BM;GOLDSTEIN, A
测定了兔和大鼠脊髓背侧、腹侧和背根神经节中免疫反应性强啡肽（ir-dynorphin）的分布。两种动物的浓度均以脊髓背部最高，脊髓腹部居中，背根神经节含量较低。在兔脊髓和背根神经节的检查节段（椎骨C2-S3）中，ir-dynorphin的水平相对均匀。凝胶渗透色谱法对兔脊髓背、腹侧和背根神经节的提取物进行分析，发现至少有3种不同分子大小的免疫活性成分存在于3种结构中。大鼠单侧或双侧多次脊背根切断术（C5-T1椎体）对脊髓内ir-dynorphin水平无影响。在脊髓内，这种神经肽似乎包含在短轴突神经元中。这种强效的阿片肽可能参与脊髓感觉信息的加工。
The distribution of immunoreactive dynorphin (ir-dynorphin) was determined in dorsal and ventral aspects of spinal cord and in dorsal root ganglia of rabbit and rat. Concentrations are highest in dorsal cord, with intermediate levels in ventral cord and low levels in dorsal root ganglia of both species. Levels of ir-dynorphin are relatively uniform over examined segments (vertebrae C2-S3) of rabbit spinal cord and dorsal root ganglia. Gel permeation chromatography of extracts from rabbit dorsal and ventral spinal cord and dorsal root ganglia revealed at least 3 immunoreactive components of differing molecular size in all 3 structures. Multiple unilateral or bilateral dorsal rhizotomy (vertebrae C5-T1) in rat did not affect levels of ir-dynorphin in spinal cord. Within the spinal cord, the neuropeptide appears to be contained in short-axoned neurons. This potent opioid peptide may participate in the processing of sensory information in spinal cord.