A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes
A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes
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DOI：
10.1128/jvi.00481-06
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Leopold, Philip L.
中科院分区：
文献类型：
--
作者：
Kelkar, Samir;De, Bishnu P.;Leopold, Philip L.
在感染过程中，腺病毒相关病毒（AAV）作为病毒基因组载体运输程序的一部分，经历微管依赖的逆行运输到细胞核。采用微管结合实验来评估细胞质动力蛋白介导AAV与微管相互作用的假设。总细胞微管相关蛋白（MAPs）的存在以核苷酸依赖的方式增强了AAV血清型2 （AAV2）的结合，而不是细胞质动力蛋白耗尽的MAPs。过量的AAV2衣壳蛋白阻止了AAV血清型2、5和rh的微管结合。10，以及血清型5腺病毒，表明这些病毒使用类似的结合位点。
During infection, adenovirus-associated virus (AAV) undergoes microtubule-dependent retrograde transport as part of a program of vectorial transport of viral genome to the nucleus. A microtubule binding assay was used to evaluate the hypothesis that cytoplasmic dynein mediates AAV interaction with microtubules. Binding of AAV serotype 2 (AAV2) was enhanced in a nucleotide-dependent manner by the presence of total cellular microtubule-associated proteins (MAPs) but not cytoplasmic dynein-depleted MAPs. Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses.