Characteristics of probe electrospray generated from a solid needle
Characteristics of probe electrospray generated from a solid needle
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DOI：
10.1021/jp803730x
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发表时间：
2008-09-04
影响因子：
3.3
通讯作者：
Hiraoka, Kenzo
中科院分区：
文献类型：
--
作者：
Chen, Lee Chuin;Nishidate, Kentaro;Hiraoka, Kenzo
探针电喷雾电离（PESI）是近年来发展起来的一种新型电喷雾电离技术，它是由一根固体针产生的，而不是通过毛细管产生的。本文在喷雾电流测量、光学显微镜和PESI质谱分析的基础上，研究了探针电喷雾电离特性。在实验中，固体针沿垂直轴上下移动，当针尖接触液体样品表面的最低位置时，少量样品反复加载到针上。施加高压后，在固体针探针的尖端形成液滴，其大小由针尖的大小决定。如喷雾电流所示，液体流向针尖的速率取决于施加到针上的电压以及负载的液体量。可以保持稳定的电喷雾，直到液体样品的总消耗。千赫兹电流脉动发生在样品过载到针的情况下。研究了施加电压和液体流速对PESI质谱的影响。
Probe electrospray ionization (PESI) has recently been developed, in which the electrospray was generated from a solid needle instead of by using a capillary. In this paper, the characteristics of probe electrospray ionization were studied based on the measurement of spray current, optical microscopy, and PESI mass spectrometry. In the experiment, the solid needle was moved up and down a vertical axis, and a small amount of sample was repeatedly loaded to the needle when the tip of the needle touched the surface of the liquid sample at the lowest position. After the application of high voltage, a liquid droplet was formed on the tip of the solid needle probe, with its size was determined by the size of the needle tip. The liquid flow rate to the tip, as indicated by the spray current, depends on the voltage applied to the needle as well as the loaded liquid amount. Stable electrospray can be maintained until the total consumption of liquid sample. The kilohertz current pulsation takes place in the case of overloading the sample to the needle. The influences of the applied voltage and the liquid flow rate on the PESI mass spectra were also examined.