Sustained BK Viruria as an Early Marker for the Development of BKV-Associated Nephropathy: Analysis of 4128 Urine and Serum Samples
Sustained BK Viruria as an Early Marker for the Development of BKV-Associated Nephropathy: Analysis of 4128 Urine and Serum Samples
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DOI：
10.1097/tp.0b013e3181aa8f62
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发表时间：
2009-07-15
期刊：
影响因子：
6.2
通讯作者：
Reinke, Petra
中科院分区：
文献类型：
--
作者：
Babel, Nina;Fendt, Juliane;Reinke, Petra
背景资料。BKV激活在BKV相关性肾病(BKVAN)的发生发展中起重要作用。由于缺乏有效的治疗方法，早期诊断BKV再激活是预防BKVAN的关键。BKV停留在尿路上皮细胞中，首先从尿液中排泄出来，然后才能在血浆中检测到。本研究分析了BK病毒血症对BK病毒血症发展的预测价值及其对BKVAN早期预测的可能优势。应用实时定量聚合酶链式反应对433例肾移植患者尿液和血清标本进行BKV检测，其中横断面433例，纵向233例。前瞻性纵向分析包括移植后第一年的七项测量。血清和尿液中BKV阳性率分别为7%和19%。持续性BK病毒血症诊断BK病毒血症的敏感性为100%，特异性为94%，且与BK负荷水平显著高于一过性BK血症(P
Background. BKV reactivation plays the causative role in the development of BKV-associated nephropathy (BKVAN). Because of the lack of effective therapy, early diagnosis of BKV reactivation is paramount for the prevention of BKVAN. Resting in uroepithelial cells, BKV is excreted first in urine before it call be detected in plasma. The present study analyzed predictive value of BK viruria for the development of BK viremia and its possible advantage for the early BKVAN prediction.Methods. Total of 4128 urine and serum samples obtained from renal transplant patients were analyzed for BKV positivity by real-time polymerase chain reaction in 433 patients in cross-sectional and in 233 patients in longitudinal manner, respectively. The prospective longitudinal analysis included seven measurements during the first posttransplant year.Results. A total of 7% and 19% patients were positive for BKV in serum and Urine, respectively. Sustained BK viruria showed sensitivity of 100% and specificity of 94% for BK viremia and was associated with significantly higher level of BK load than the patients with transient viruria (P