Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes
Transdifferentiation of blood-derived human adult endothelial progenitor cells into functionally active cardiomyocytes
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DOI：
10.1161/01.cir.0000051460.85800.bb
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发表时间：
2003-02-25
期刊：
影响因子：
37.8
通讯作者：
Dimmeler, S
中科院分区：
文献类型：
--
作者：
Badorff, C;Brandes, RP;Dimmeler, S
除了促进血管生成外，细胞治疗可能是心脏再生的一种方法。最近的研究表明，祖细胞可以转分化成其他谱系。然而，内皮祖细胞（EPCs）的转分化潜能是unknown.Methods和Results-EPCs从健康成人或冠状动脉疾病（CAD）患者的外周血单个核细胞培养内皮细胞培养基和生长因子。3天后，>95%的贴壁细胞在功能和表型上是EPC。然后将二乙酰化LDL标记的EPC与大鼠心肌细胞共培养6天，导致EPC细胞长度和大小显著增加，形成心肌细胞样形态。健康成人（n=15）和冠心病患者（n=14）的EPCs分别为9.94 ± 1.39%和5.04 ± 1.09%（P
Background-Further to promoting angiogenesis, cell therapy may be an approach for cardiac regeneration. Recent studies suggest that progenitor cells can transdifferentiate into other lineages. However, the transdifferentiation potential of endothelial progenitor cells (EPCs) is unknown.Methods and Results-EPCs were obtained from peripheral blood mononuclear cells of healthy adults or coronary artery disease (CAD) patients by cultivating with endothelial cell medium and growth factors. After 3 days, >95% of adherent cells were functionally and phenotypically EPCs. Diacetylated LDL-labeled EPCs were then cocultivated with rat cardiomyocytes for 6 days, resulting in significant increases of EPC cell length and size to a cardiomyocyte-like morphology. Biochemically, 9.94+/-1.39% and 5.04+/-1.09% of EPCs from healthy adults (n=15) or CAD patients (n=14, P