Pausing and premature termination of human RNA polymerase II during transcription of adenovirus in vivo and in vitro.
Pausing and premature termination of human RNA polymerase II during transcription of adenovirus in vivo and in vitro.
复制标题
体内和体外腺病毒转录过程中人 RNA 聚合酶 II 的暂停和提前终止。
DOI：
10.1073/pnas.81.19.5931
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Chen-Kiang,S
中科院分区：
文献类型：
--
作者：
Maderious,A;Chen-Kiang,S
腺病毒2型的主要晚期转录单位已成为研究真核生物转录的模型。我们报道，暂停和提前终止是RNA聚合酶II对该转录单位的固有转录。在体内和在分离的核中，转录在起始点附近的离散位置暂停，并可能在第175位核苷酸和可能的第120位核苷酸提前终止。提前终止的RNA与转录复合体无关，并在体内积累在细胞核中，而暂停的RNA仍与转录复合体相关，并延伸成全长转录本。停顿也在可溶系统中的转录复合体中复制。5，6-Dichloro-1-beta-D-ribofuranosylbenzimidazole增强了暂停，但不是过早终止，其作用是可逆的。所提出的腺病毒2型中175位核苷酸的提前终止位点与大肠杆菌噬菌体lambda中的TR1位点具有序列同源性。
The major late transcriptional unit of adenovirus type 2 has served as a model for studying transcription in eukaryotes. We report that pausing and premature termination are intrinsic to the transcription of this transcriptional unit by RNA polymerase II. In vivo and in isolated nuclei, transcription pauses at discrete sites proximal to the initiation site and can prematurely terminate at nucleotide 175 and possibly also at nucleotide 120. The prematurely terminated RNAs are not associated with the transcription complexes and accumulate in the cell nucleus in vivo, whereas paused RNAs remain associated with the transcription complexes and elongate into full-length transcripts. Pausing is also reproduced in the transcription complexes in a soluble system. 5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole enhances pausing but not premature termination, and its action is reversible. The proposed premature termination site at nucleotide 175 in adenovirus type 2 bears sequence homology to the tR1 site in coliphage lambda.