The production of biofuels from carbonated beverages
The production of biofuels from carbonated beverages
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DOI：
10.1016/j.apenergy.2012.02.054
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发表时间：
2012-12-01
期刊：
影响因子：
11.2
通讯作者：
Mitchell, Robert J.
中科院分区：
文献类型：
--
作者：
Dwidar, Mohammed;Lee, Siseon;Mitchell, Robert J.
通常销售的碳酸饮料通常含有110g/L的糖。因此，这项研究评估了来自全球两家大型分销商和一家当地分销商的四种产品作为发酵糖源的使用情况，其中包括两种可乐、一种健怡可乐和一种柠檬酸橙口味的饮料。运动发酵单胞菌ZM4能够发酵所含的蔗糖、果糖和葡萄糖，在添加后约10h内将三种糖全部消耗。除减肥饮料外，每种饮料的结果都是相似的，最终乙醇浓度约为25g/L，平均生产率约为2g*L(-1)h(-1)，平均得率为0.43g乙醇/g葡萄糖。使用一种外加果糖-葡萄糖混合物的减肥饮料，我们证明该饮料对培养物没有抑制作用或毒性。随后对三种梭状芽胞杆菌菌株进行的实验表明，这些饮料也可以用于发酵过程中产生丁醇和丁酸。与运动梭菌ZM4一样，乙酰丁酸梭菌ATCC824和别氏梭菌NCIMB8052都能发酵这三种糖，最终丁醇浓度分别为5.3g/L和8.7g/L。相反，酪丁酸梭菌ATCC 25755不能利用蔗糖作为碳源。虽然仅限于单糖、果糖和葡萄糖，但丁酸的浓度为15.4g/L，丁酸盐的得率为0.43g/g葡萄糖，仅略低于理论最大值0.47.这项研究说明了在生物燃料行业使用废弃或过期的碳酸饮料的潜力。(C)2012爱思唯尔有限公司。保留所有权利。
Commonly sold carbonated beverages typically contain 110 g/L of sugar. Consequently, this study evaluated the use of four products, including two colas, one diet cola and one lemon-lime flavored beverage from two large distributors world-wide and one local distributor, as fermentative sugar sources. Zymomonas mobilis ZM4 cultures were able to ferment the sugars present, which included sucrose, fructose and glucose, consuming all three completely within about 10 h after addition. The results from each of the beverages tested were similar, except for the diet beverage, with a final ethanol concentrations of around 25 g/L, an average productivity of approximately 2 g*L(-1)h(-1) and average yields of 0.43 g ethanol/g glucose. Using a diet beverage with a fructose-glucose mixture added extraneously, we demonstrated that the beverages were not inhibitory or toxic to the cultures. Subsequent experiments with three clostridial strains showed that these beverages can also be used within fermentative processes to generate butanol and butyric acid. As with Z. mobilis ZM4, Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB 8052 were capable of fermenting all three sugars and had final butanol concentrations of 5.3 and 8.7 g/L, respectively. In contrast, Clostridium tyrobutyricum ATCC 25755 is unable to utilize sucrose as a carbon source. Although limited to only the monosaccharides fructose and glucose, the butyric acid concentration was 15.4 g/L and had a yield of 0.43 g butyrate/g glucose, which is only slightly below the theoretical maximum of 0.47. This study illustrates the potential of using wasted or expired carbonated beverages in the biofuels industry. (c) 2012 Elsevier Ltd. All rights reserved.